Tao Y, Hu L, Li S, Bai Y, Liu Q, Jin Y, Wei D, Luo Z
Department of Nephrology, West China Hospital; Department of Immunology, College of Pre-clinical and Forensic Medicine, West China Center of Medical Sciences, Sichuan University, China.
Transplant Proc. 2009 Dec;41(10):4373-5. doi: 10.1016/j.transproceed.2009.09.073.
Our aim was to explore the effects of the anti-allergic and antifibrotic agent tranilast on chronic cyclosporine (CsA) nephrotoxicity in rats.
Eighteen Sprague-Dawley rats were randomized to be given the following daily treatments by gavage for 4 weeks: (1) controls, olive oil; (2) CsA group, CsA 25 mg/kg; (3) CsA plus tranilast group, CsA 25 mg/kg and tranilast 400 mg/kg. We examined the body weights and the effects of tranilast on histopathology, macrophage (Mvarphi) infiltration, and expression of osteopontin (OPN).
The administration of tranilast improved body weight gain by CsA-treated rats (232 +/- 24 vs 203 +/- 6 g; P < .05). This treatment reduced the expression of OPN protein and infiltration of macrophages (9.14 +/- 2.7 vs 22.44 +/- 5.68 ED-1 positive cells per high power field, P < .05). Furthermore compared with the CsA alone group, it ameliorated tubulointerstitial fibrosis scores (1.18 +/- 0.08 vs 2.57 +/- 0.21, P < .05).
Tranilast attenuated tubulointerstitial fibrosis through decreased expression of OPN protein and macrophage infiltration, showing renal protective effects in rats with chronic CsA nephrotoxicity.
我们的目的是探讨抗组胺和抗纤维化药物曲尼司特对大鼠慢性环孢素（CsA）肾毒性的影响。
18只Sprague-Dawley大鼠被随机分为三组，每天经口灌胃给药4周：（1）对照组，给予橄榄油；（2）CsA组，给予CsA 25 mg/kg；（3）CsA加曲尼司特组，给予CsA 25 mg/kg和曲尼司特400 mg/kg。我们检测了体重以及曲尼司特对组织病理学、巨噬细胞（Mφ）浸润和骨桥蛋白（OPN）表达的影响。
给予曲尼司特可改善CsA处理大鼠的体重增加（232±24 vs 203±6 g；P <.05）。这种处理降低了OPN蛋白的表达和巨噬细胞的浸润（每高倍视野9.14±2.7 vs 22.44±5.68个ED-1阳性细胞，P <.05）。此外，与单独使用CsA组相比，它改善了肾小管间质纤维化评分（1.18±0.08 vs 2.57±0.21，P <.05）。
曲尼司特通过降低OPN蛋白表达和巨噬细胞浸润减轻肾小管间质纤维化，对慢性CsA肾毒性大鼠显示出肾脏保护作用。
