Division of Applied Life Science, Graduate School of Gyeongsang National University, Jinju, Republic of Korea.
Theriogenology. 2010 Mar 15;73(5):638-44. doi: 10.1016/j.theriogenology.2009.10.020. Epub 2009 Dec 14.
We investigated the sperm characteristics of four cloned male cats (Felis catus) to assess their reproductive potential. Fresh and frozen-thawed sperm were assessed for motility, viability, and morphology, and their functional competence was evaluated by in vitro fertilization (IVF) of domestic cat oocytes. All fresh semen characteristics varied among cats and collection times. Sperm concentration (x 10(6)/mL) of Cat A (512+/-140, range 368 to 685) was significantly higher, whereas that of Cat C (335+/-92, range 274 to 469) was significantly lower than that of Cloned B (459+/-159, range 336 to 510) and control cats (680+/-452, range 360 to 479). After thawing, motility and progressive motility of sperm from Cat B were significantly lower than that of the other cloned and control cats. The curvilinear, straight line, and average path velocities of sperm from Cat B were significantly higher, whereas the straightness was lower, than that of the other cloned and control cats. Frozen sperm from Cats A, B, and C successfully fertilized oocytes (cleavage=74.4%, 71.4%, and 86.2%, respectively) and produced embryos that developed to the blastocyst stage after IVF/In vitro culture (IVC) (34.4%, 26.7%, and 48.0%) at frequencies similar to the cleavage rate (82.0%) and blastocyst rate (43.9%) obtained with sperm from the control male. In conclusion, seminal characteristics of cloned male cats did not differ markedly from those of our noncloned, control male cats.
我们研究了四只克隆雄性猫（Felis catus）的精子特征，以评估它们的生殖潜力。对新鲜和冷冻解冻的精子进行了活力、存活和形态评估，并通过体外受精（IVF）对家猫卵母细胞评估了其功能能力。所有新鲜精液特征在猫和采集时间之间均存在差异。猫 A 的精子浓度（x 10(6)/mL）（512+/-140，范围 368 至 685）显著较高，而猫 C 的精子浓度（335+/-92，范围 274 至 469）显著低于克隆 B（459+/-159，范围 336 至 510）和对照猫（680+/-452，范围 360 至 479）。解冻后，猫 B 的精子活力和向前运动能力显著低于其他克隆和对照猫的精子。猫 B 的精子曲线、直线和平均路径速度显著较高，而直线度显著较低。猫 A、B 和 C 的冷冻精子成功受精了卵母细胞（分别为 74.4%、71.4%和 86.2%），并在 IVF/体外培养（IVC）后产生了发育到囊胚阶段的胚胎（分别为 34.4%、26.7%和 48.0%），其受精率（82.0%）和囊胚率（43.9%）与从对照雄性获得的精子相似。总之，克隆雄性猫的精液特征与我们的非克隆对照雄性猫的特征没有明显差异。
