Tecirlioglu R Tayfur, Cooney Melissa A, Korfiatis Natasha A, Hodgson Renee, Williamson Mark, Downie Shara, Galloway David B, French Andrew J
Monash Immunology and Stem Cell Laboratories (MISCL), Monash Science Technology Research and Innovation Precinct (STRIP), Building 75, Monash University, Wellington Road, Clayton, Victoria 3800, Australia.
Theriogenology. 2006 Jun;65(9):1783-99. doi: 10.1016/j.theriogenology.2005.09.033. Epub 2005 Nov 14.
In this comparative study, reproductive parameters and semen characteristics of cloned bulls (n = 3) derived from somatic cell nuclear transfer (SCNT) were compared to their original cell donor Holstein-Friesian (n = 2) bulls from the same enterprise to assess the differences in reproductive potential between a donor bull and its clones. The parameters evaluated included motility of fresh, frozen-thawed and Percoll-treated frozen-thawed spermatozoa, as well as in vitro fertilization (IVF) ability, embryo quality, birth and survival of calves following IVF and embryo transfer with frozen-thawed semen. With fresh semen, spermatozoa from one cloned bull had lower motility than its donor. Cloned bulls had higher velocity parameters in fresh semen, but those effects were not obvious in frozen-thawed or frozen-thawed semen selected with a Percoll gradient. Semen collected from cloned bulls had significantly higher IVF rates compared to donors; however, embryo development per cleaved embryo or quality of blastocysts did not differ between donors and cloned bulls. Pregnancy and live offspring rates from one donor and its cloned bull did not differ between fresh (40%, 16/40 versus 46%, 17/37) and vitrified/thawed (13%, 2/16 versus 25%, 4/16) embryo transfer following IVF. A total of 26 calves were obtained from genotypically identical donor and cloned bulls with no signs of phenotypical abnormalities. These preliminary results suggested that the physiology of surviving postpubertal cloned bulls and quality of collected semen had equivalent reproductive potential to their original cell donor, with no evidence of any deleterious effects in their progeny.
在这项比较研究中，将通过体细胞核移植（SCNT）获得的克隆公牛（n = 3）的繁殖参数和精液特征与其来自同一企业的原始细胞供体荷斯坦 - 弗里生公牛（n = 2）进行比较，以评估供体公牛与其克隆体之间繁殖潜力的差异。评估的参数包括新鲜、冻融和经Percoll处理的冻融精子的活力，以及体外受精（IVF）能力、胚胎质量、使用冻融精液进行IVF和胚胎移植后犊牛的出生和存活情况。对于新鲜精液，一头克隆公牛的精子活力低于其供体。克隆公牛的新鲜精液具有较高的速度参数，但在冻融或经Percoll梯度选择的冻融精液中这些影响并不明显。与供体相比，从克隆公牛采集的精液具有显著更高的IVF率；然而，供体和克隆公牛之间每个分裂胚胎的胚胎发育或囊胚质量没有差异。在IVF后的新鲜（40%，16/40对46%，17/37）和玻璃化/解冻（13%，2/16对25%，4/16）胚胎移植中，一头供体及其克隆公牛的妊娠率和活产后代率没有差异。从基因相同的供体和克隆公牛共获得了26头犊牛，没有任何表型异常的迹象。这些初步结果表明，存活的青春期后克隆公牛的生理状况和采集精液的质量与其原始细胞供体具有同等的繁殖潜力，且没有证据表明其后代有任何有害影响。
