State Key Lab of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China.
Bioresour Technol. 2010 Apr;101(8):2643-50. doi: 10.1016/j.biortech.2009.10.068. Epub 2009 Dec 14.
The composition and dynamics of sulfate-reducing bacteria (SRB) during the waterflooding process of Daqing Oilfield were investigated in this study. PCR-DGGE analysis indicated that the microbial communities were significantly different in each treatment unit, and the dominant members were mainly close to Clostridium sp., Thauera sp., Hydrogenophaga sp., Pseudomonas sp., Eubacterium sp. and Arcobacter sp. However, the members belonging to SRB were relatively few and mainly consisted of Desulfovibrio sp. and Desulfovibrio profundus. According to APS (adenosine-5'-phosphosulfate reductase) gene clone library and sequence analysis, it was found that SRB mainly belonged to Proteobacteria and Deltaproteobacteria. In the library, the genus Desulfovibrio of Deltaproteobacteria was about 65.2% while genus Desulfomonas, Desulfomicrobium, Desulfohalobium, Desulfonatronum, Desulfbulbus, Desulforhopalus, Desulfnema and Desulforhopalus were occupied nearly 34.8%. It was also proved that most of SRB in the oil field were enriched in the ground treatment unit and they are not in the liquid of oil well.
本研究调查了大庆油田注水过程中硫酸盐还原菌（SRB）的组成和动态。PCR-DGGE 分析表明，每个处理单元的微生物群落差异显著，优势成员主要接近梭菌属、陶厄氏菌属、噬氢菌属、假单胞菌属、真杆菌属和弧菌属。然而，属于 SRB 的成员相对较少，主要由脱硫弧菌和脱硫深度菌组成。根据 APS（腺苷-5'-磷酸硫酸盐还原酶）基因克隆文库和序列分析，发现 SRB 主要属于变形菌门和δ-变形菌门。在文库中，δ-变形菌门的脱硫弧菌属约占 65.2%，而脱硫单胞菌属、脱硫微菌属、脱硫咸单胞菌属、脱硫硝杆菌属、脱硫胞菌属、脱硫螺菌属、脱硫奈氏菌属和脱硫纤维菌属则占近 34.8%。这也证明了油田中的大多数 SRB 都富集在地面处理单元中，而不是在油井液体中。
