Department of Pathology, The School of Stomatology, Shandong University, Jinan, Shandong 250012, People's Republic of China.
Oral Oncol. 2010 Jan;46(1):65-9. doi: 10.1016/j.oraloncology.2009.11.003. Epub 2009 Dec 14.
The aim of this study was to examine the Kif2a expression and its role in tumor progression, invasion and metastasis in squamous cell carcinoma of the oral tongue (SCCOT). The study included 44 cases of primary tumor and the corresponding adjacent tissues, 20 cases of primary tumor with lymph node metastasis. Immunohistochemistry was used to observe the Kif2a expression and its correlation with clinicopathologic factors in oral tongue cancer. The immunohistochemistry showed that Kif2a expression was stronger in oral tongue cancer tissues than in paired adjacent tissues (P<0.01), and the higher expression of Kif2a was also significantly associated with lymph node metastasis (P<0.01), tumor clinical stage (P<0.01). In addition, in vitro results from transwell chamber assay showed that Tca8113 cells transfected with Kif2a-siRNA had a decreased migratory ability (P<0.01) compared to nonsense-siRNA-transfected cells. Therefore we speculate the overexpression of Kif2a might be involved in the progression, invasion and metastasis of SCCOT and Kif2a should be as a predictor for prognosis.
本研究旨在探讨 Kif2a 在口腔舌鳞癌（SCCOT）中的表达及其在肿瘤进展、侵袭和转移中的作用。该研究纳入了 44 例原发性肿瘤及其相应的相邻组织，20 例原发性肿瘤伴淋巴结转移。免疫组织化学用于观察 Kif2a 在口腔舌癌中的表达及其与临床病理因素的相关性。免疫组化结果显示，Kif2a 在口腔舌癌组织中的表达强于配对相邻组织（P<0.01），Kif2a 的高表达也与淋巴结转移（P<0.01）、肿瘤临床分期（P<0.01）显著相关。此外，体外转染实验结果显示，与 nonsense-siRNA 转染细胞相比，转染 Kif2a-siRNA 的 Tca8113 细胞迁移能力降低（P<0.01）。因此，我们推测 Kif2a 的过表达可能参与 SCCOT 的进展、侵袭和转移，Kif2a 可作为预后的预测因子。
