Zhang Kailiang, Jiao Kangli, Xing Zhankui, Zhang Li, Yang Juan, Xie Xiaodong, Yang Lan
School of Stomatology, Lanzhou University Lanzhou, China ; Stomatological Hospital of Lanzhou University Lanzhou, China ; Research Institute of Stomatology, Lanzhou University Lanzhou, China ; Department of Oral and Maxillofacial Surgery, Second Hospital of Lanzhou University Lanzhou, China.
School of Stomatology, Lanzhou University Lanzhou, China ; Stomatological Hospital of Lanzhou University Lanzhou, China ; Research Institute of Stomatology, Lanzhou University Lanzhou, China.
Int J Clin Exp Pathol. 2014 Oct 15;7(11):7360-77. eCollection 2014.
Apoptosis-related protein B-cell lymphoma-extra large (Bcl-xL) has a crucial role in the control of cell death through its inhibition of apoptosis. This study was designed to investigate the expression of Bcl-xL in relation to the development of tongue carcinoma and whether it has potential as a marker for the clinical diagnosis of tongue carcinoma and as a therapeutic target to evaluate the dynamic of tongue carcinoma progression. A statistical analysis of 100 cases oral tongue carcinoma tissue specimens were performed using pathological grading and clinical TNM staging, and 14 cases corresponding non-tumor tissues as control. The changes in Bcl-xL mRNA expression between different pathological grades and clinical TNM stages of tissue were analyzed by RT-PCR. Additionally, immunohistochemical SP method and Western blot assays were employed to detect changes in Bcl-xL protein expression in different tongue carcinoma tissues. The results showed the expression of Bcl-xL was significantly higher in tongue carcinoma tissues than in normal tongue tissues and was positively associated with the degree of differentiation and the clinical TNM staging, but negatively correlated with the degree of malignancy of the tumor. There was higher expression of Bcl-xL in oral tongue squamous cell carcinoma (OTSCC) tissues compared with oral tongue adenocarcinoma (OTA) tissues, but Bcl-xL expression in tissue with lymph node metastasis was significantly higher than that without lymph node metastasis. Thus, Bcl-xL overexpression may be closely related to the dynamic of the pathogenesis and development of tongue carcinoma. It may be a useful marker for clinical diagnosis and an aid to evaluating the efficacy of therapeutics in tongue carcinoma.
凋亡相关蛋白B细胞淋巴瘤-特大（Bcl-xL）通过抑制细胞凋亡在细胞死亡控制中发挥关键作用。本研究旨在探讨Bcl-xL在舌癌发生发展中的表达情况，以及它是否有潜力作为舌癌临床诊断的标志物和评估舌癌进展动态的治疗靶点。对100例口腔舌癌组织标本进行病理分级和临床TNM分期的统计分析，并以14例相应的非肿瘤组织作为对照。采用RT-PCR分析不同病理分级和临床TNM分期组织中Bcl-xL mRNA表达的变化。此外，采用免疫组织化学SP法和Western blot检测不同舌癌组织中Bcl-xL蛋白表达的变化。结果显示，Bcl-xL在舌癌组织中的表达明显高于正常舌组织，且与分化程度和临床TNM分期呈正相关，但与肿瘤恶性程度呈负相关。与口腔舌腺癌（OTA）组织相比，口腔舌鳞状细胞癌（OTSCC）组织中Bcl-xL的表达更高，但有淋巴结转移的组织中Bcl-xL的表达明显高于无淋巴结转移的组织。因此，Bcl-xL的过表达可能与舌癌的发病机制和发展动态密切相关。它可能是临床诊断的有用标志物，并有助于评估舌癌的治疗效果。
