抗SPARC寡肽可抑制小鼠激光诱导的脉络膜新生血管。
Anti-SPARC oligopeptide inhibits laser-induced CNV in mice.
作者信息
Uehara Hironori, Luo Ling, Simonis Jacquelyn, Singh Nirbhai, Taylor Ethan Will, Ambati Balamurali K
机构信息
John A. Moran Eye Center, Department of Ophthalmology and Visual Sciences, University of Utah, 65 Mario Capecchi Dr., Salt Lake City, UT 84132, USA.
出版信息
Vision Res. 2010 Mar 31;50(7):674-9. doi: 10.1016/j.visres.2009.12.003. Epub 2009 Dec 22.
Abstract
It is known that SPARC gates VEGF-A signal transduction towards KDR, the primary angiogenic VEGF receptor. We sought to determine whether inhibition of SPARC activity using anti-SPARC peptide could inhibit laser-induced CNV by promoting binding of VEGF-A to FLT-1. We created anti-SPARC l-peptide and retro-inverso anti-SPARC d-peptide. Anti-SPARC peptides or PBS were injected intravitreally 1day before or after laser induction. Intravitreal injection of anti-SPARC l-peptide 1day before laser induction promotes FLT-1 phosphorylation and inhibited laser-induced CNV and anti-SPARC d-peptide had no effect. Injection 1day after laser injury did not affect size of laser-induced CNV. Inhibition of SPARC activity could be complementary to existing anti-CNV therapy.
摘要
已知SPARC调控血管内皮生长因子A（VEGF-A）向主要血管生成性VEGF受体激酶插入域受体（KDR）的信号转导。我们试图确定使用抗SPARC肽抑制SPARC活性是否可通过促进VEGF-A与Fms样酪氨酸激酶1（FLT-1）结合来抑制激光诱导的脉络膜新生血管（CNV）。我们制备了抗SPARC l肽和反向抗SPARC d肽。在激光诱导前或后1天经玻璃体腔注射抗SPARC肽或磷酸盐缓冲盐水（PBS）。激光诱导前1天经玻璃体腔注射抗SPARC l肽可促进FLT-1磷酸化并抑制激光诱导的CNV，而抗SPARC d肽则无作用。激光损伤后1天注射对激光诱导的CNV大小无影响。抑制SPARC活性可能是现有抗CNV治疗的补充。
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