State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China.
Talanta. 2010 Jan 15;80(3):1264-8. doi: 10.1016/j.talanta.2009.09.019.
To our knowledge, we report the first fluorescence aptasensor for detecting human neutrophil elastase (HNE) in homogeneous solution. The biosensor contains a short DNA scrambled sequence strand (SS) complementary to part of the aptamer sequence or the loop of molecular beacon (MB). The aptamer-HNE recognition event involves competition between the molecular beacon and loose HNE aptamer for the binding the short DNA strand. The new biosensor can detect as little as 0.34nM of HNE, and the response is linear in the tested concentration range of 0.34-68nM with the detection limit of 47pM.
据我们所知，我们报道了首个用于在均相溶液中检测人中性粒细胞弹性蛋白酶（HNE）的荧光适体传感器。该生物传感器包含与适体序列的一部分或分子信标（MB）的环互补的短 DNA 随机序列链（SS）。适体-HNE 识别事件涉及分子信标和游离 HNE 适体与短 DNA 链的竞争结合。新的生物传感器可以检测低至 0.34nM 的 HNE，并且响应在测试的浓度范围内 0.34-68nM 呈线性，检测限为 47pM。
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