State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, PR China.
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, PR China; International Joint Laboratory on Food Safety, School of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, PR China.
Food Chem. 2021 Apr 16;342:128303. doi: 10.1016/j.foodchem.2020.128303. Epub 2020 Oct 6.
An ultrasensitive, rapid, and specific method for Ochratoxin A (OTA) detection was designed using complementary sequence to aptamer as a target of molecular beacon (MB). The designed loop structure of the MB has the same sequence as the aptamer with a complementary DNA (cDNA) which translates the level of the target into a measurable response. The presence of the target holds aptamer at the corresponding amount and the additional cDNAs are consumed by unbound aptamers which avails free cDNAs that resulting in fluorescence rising due to unfolding of MBs. Under the optimized conditions, the fluorescence intensity increased linearly with OTA concentration over the range of 10 pg mL-1 µg mL with the detection limit of 0.247 pg mL. The application of this assay in wheat sample in comparison with HPLC-MS/MS method, demonstrated that the new assay could be a potential sensing platform for OTA detection.
本研究设计了一种基于互补序列适配体作为分子信标（MB）靶标的超灵敏、快速、特异的赭曲霉毒素 A（OTA）检测方法。MB 的设计环结构与具有互补 DNA（cDNA）的适配体具有相同的序列，该 cDNA 将靶标水平转化为可测量的响应。靶标的存在使适配体保持在相应的量，并且未结合的适配体消耗额外的 cDNA，从而利用游离的 cDNA 导致 MB 展开，荧光上升。在优化条件下，荧光强度随 OTA 浓度在 10 pg mL-1 µg mL-1 范围内呈线性增加，检测限为 0.247 pg mL。与 HPLC-MS/MS 方法相比，该方法在小麦样品中的应用表明，该新方法可能是 OTA 检测的潜在传感平台。
