Laboratoire Interfaces-Traitements-Organisation et Dynamique des Systèmes (ITODYS), Université Paris-Diderot, associé au CNRS, UMR 7086, 15 rue Jean-Antoine de Baïf, 75205 Paris, France.
Talanta. 2010 Jan 15;80(3):1318-25. doi: 10.1016/j.talanta.2009.09.025.
We developed a method to graft a tripeptide (glutathione) onto 5-hydroxy-1,4-naphthoquinone, an electropolymerizable molecule. The resulting thin conducting polymer presents a well-defined and stable electroactivity in neutral buffered solution, due to the embedded quinone group, and is able to covalently graft amino-modified DNA probe strands. It is shown that the bioelectrode presents positive current change following DNA hybridization. This makes a "signal-on" direct electrochemical DNA sensor. The results were obtained with low target concentration (50nM) and the selectivity is excellent as a single-mismatch sequence can be discriminated from the full-complementary target.
我们开发了一种将三肽（谷胱甘肽）接枝到 5-羟基-1,4-萘醌的方法，5-羟基-1,4-萘醌是一种可电化学聚合的分子。所得的薄导电聚合物由于嵌入的醌基团而在中性缓冲溶液中呈现出明确且稳定的电化学活性，并且能够共价接枝氨基修饰的 DNA 探针链。结果表明，生物电极在 DNA 杂交后呈现出正电流变化。这使得该生物电极成为一种“信号开启”的直接电化学 DNA 传感器。在低靶浓度（50nM）下获得了结果，并且由于单错配序列可以与完全互补的靶序列区分开来，因此具有出色的选择性。
