Department Microbial and Molecular Pathogenesis, Texas A&M University Health Sciences Center, 471 Reynolds Medical Building, College Station, TX 77843, USA.
Tuberculosis (Edinb). 2009 Dec;89 Suppl 1(0 1):S15-7. doi: 10.1016/S1472-9792(09)70006-X.
The incidence of extrapulmonary tuberculosis is increasing, possibly due to the high frequency of co-infection with HIV. Extrapulmonary infections complicate diagnosis, have higher mortality rates and are more difficult to treat. Insight into the mechanisms involved in extrapulmonary spread of tuberculosis is critical to improving management. We set out to better understand extrapulmonary spread kinetics in mice and guinea pigs as well as the effects of infectious dose. We found that extrapulmonary spread occurs at a discrete time point when infected by low-dose aerosol, but at high-dose aerosol it occurs within the first 24h. The ability to follow tuberculosis in real-time during infection would allow us to better address the mechanisms involved. We found that mycobacteria can be optically imaged after pulmonary infection in the mouse lung, suggesting that this technology could be applied to study of extrapulmonary spread of tuberculosis.
肺外结核的发病率正在上升，这可能是由于与 HIV 合并感染的频率较高所致。肺外感染使诊断变得复杂，死亡率更高，治疗也更困难。深入了解结核病肺外传播的机制对于改善管理至关重要。我们着手更好地了解小鼠和豚鼠的肺外传播动力学以及感染剂量的影响。我们发现，当感染低剂量气溶胶时，肺外传播发生在一个离散的时间点，但当感染高剂量气溶胶时，它发生在最初的 24 小时内。在感染过程中实时跟踪结核病的能力将使我们能够更好地了解所涉及的机制。我们发现，在小鼠肺部感染后可以对分枝杆菌进行光学成像，这表明该技术可应用于研究结核病的肺外传播。
