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RBP-Jkappa（CSL）蛋白与卡波济肉瘤相关疱疹病毒 ORF47（gL）基因启动子的结合是由 ORF50 蛋白进行反式激活的关键但非充分决定因素。

Binding of RBP-Jkappa (CSL) protein to the promoter of the Kaposi's sarcoma-associated herpesvirus ORF47 (gL) gene is a critical but not sufficient determinant of transactivation by ORF50 protein.

机构信息

Graduate Institute of Clinical Medical Sciences, Chang-Gung University, Taoyuan, Taiwan.

出版信息

Virology. 2010 Mar 1;398(1):38-48. doi: 10.1016/j.virol.2009.11.022. Epub 2009 Dec 16.

DOI:10.1016/j.virol.2009.11.022
PMID:20006367
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2823930/
Abstract

ORF50 protein activates the KSHV lytic cycle. The promoter of an early lytic-cycle gene ORF47, encoding envelope protein gL, is activated by an interaction between ORF50 protein and RBP-Jkappa. In ORF47p only one of two sequences fitting the consensus RBP-Jkappa recognition site strongly binds RBP-Jkappa and confers a response to ORF50 protein. Flanking sequences 5' to the RBP-Jkappa binding site are required to confer a maximal response to ORF50 protein. Not all mutant ORF50 response elements in the ORF47p that are bound by RBP-Jkappa are sufficient to confer maximal ORF50 responsiveness. Four sequences containing an RBP-Jkappa site and flanking sequences characteristic of the ORF50 response element in ORF47p were identified in human DNA. All bound RBP-Jkappa, but only one responded robustly to ORF50 protein. We propose models for the possible function of ancillary sequences flanking the RBP-Jkappa-binding element which are crucial for mediating ORF50 transactivation.

摘要

ORF50 蛋白激活 KSHV 裂解周期。编码包膜蛋白 gL 的早期裂解周期基因 ORF47 的启动子被 ORF50 蛋白与 RBP-Jkappa 之间的相互作用激活。在 ORF47p 中，只有两个符合 RBP-Jkappa 识别位点共识的序列之一强烈结合 RBP-Jkappa 并赋予对 ORF50 蛋白的反应。RBP-Jkappa 结合位点 5' 侧翼序列对于赋予对 ORF50 蛋白的最大反应是必需的。并非所有与 RBP-Jkappa 结合的 ORF47p 中突变的 ORF50 反应元件都足以赋予最大的 ORF50 反应性。在人 DNA 中鉴定了四个包含 RBP-Jkappa 位点和 ORF47p 中 ORF50 反应元件特征侧翼序列的序列。所有这些序列都与 RBP-Jkappa 结合，但只有一个对 ORF50 蛋白有强烈反应。我们提出了 RBP-Jkappa 结合元件侧翼辅助序列的可能功能模型，这些序列对于介导 ORF50 反式激活至关重要。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6de0/2823930/edced93ba2a2/nihms160252f10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6de0/2823930/38f8d7423a08/nihms160252f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6de0/2823930/d006ba407bb6/nihms160252f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6de0/2823930/eadb0e607c6a/nihms160252f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6de0/2823930/0834b9e05422/nihms160252f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6de0/2823930/2ece2ad3e015/nihms160252f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6de0/2823930/db7a013f3876/nihms160252f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6de0/2823930/85e14fe0292a/nihms160252f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6de0/2823930/025eed3cbc9a/nihms160252f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6de0/2823930/8c633db149b8/nihms160252f9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6de0/2823930/edced93ba2a2/nihms160252f10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6de0/2823930/38f8d7423a08/nihms160252f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6de0/2823930/d006ba407bb6/nihms160252f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6de0/2823930/eadb0e607c6a/nihms160252f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6de0/2823930/0834b9e05422/nihms160252f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6de0/2823930/2ece2ad3e015/nihms160252f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6de0/2823930/db7a013f3876/nihms160252f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6de0/2823930/85e14fe0292a/nihms160252f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6de0/2823930/025eed3cbc9a/nihms160252f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6de0/2823930/8c633db149b8/nihms160252f9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6de0/2823930/edced93ba2a2/nihms160252f10.jpg

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A mobile functional region of Kaposi's sarcoma-associated herpesvirus ORF50 protein independently regulates DNA binding and protein abundance.卡波西肉瘤相关疱疹病毒ORF50蛋白的一个移动功能区域独立调节DNA结合和蛋白质丰度。
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The interaction between KSHV RTA and cellular RBP-Jkappa and their subsequent DNA binding are not sufficient for activation of RBP-Jkappa.卡波西肉瘤相关疱疹病毒（KSHV）的RTA与细胞RBP-Jkappa之间的相互作用及其随后的DNA结合不足以激活RBP-Jkappa。
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Virology. 2007 Mar 1;359(1):19-27. doi: 10.1016/j.virol.2006.09.032. Epub 2006 Oct 18.
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