Department of Chemistry Graduate School, Kyungpook National University, Daegu 702-701, South Korea.
Biosens Bioelectron. 2010 Mar 15;25(7):1579-86. doi: 10.1016/j.bios.2009.11.015. Epub 2009 Nov 20.
A new methodology for the fabrication of bienzymatic amperometric glucose biosensor based on the use of an organic-inorganic hybrid is presented. The fabrication involves a self-assembly directed one-pot electrochemical process. Bi-enzymes, horseradish peroxidase (HRP) and glucose oxidase (GOx) are immobilized into the porous and electroactive silica-polyaniline hybrid composite through electrochemical polymerization of N[3-(trimethoxysilyl)propyl]aniline in the presence of enzymes. The modified electrode is designated as PTMSPA/HRP-GOx. The direct electron transfer of HRP is achieved at the modified electrode. Also, the electrode exhibits excellent bio-electro-catalytic activity for the reduction of hydrogen peroxide. The response current at PTMSPA/HRP-GOx modified electrode revealed a good linear relationship with concentration of glucose range between 1 and 20mM with a response time of 7s. Thus, the modified electrode shows the combined advantages of polyaniline and silica networks through synergistic influence from the individual components. The PTMSPA assembly has shown the potential for a third generation amperometric biosensor.
提出了一种基于有机-无机杂化材料制备双酶安培葡萄糖生物传感器的新方法。该制备方法涉及自组装导向的一锅电化学过程。通过在酶存在下聚合 N-[3-(三甲氧基硅基)丙基]苯胺，将辣根过氧化物酶（HRP）和葡萄糖氧化酶（GOx）固定到多孔且具有电化学活性的硅-聚苯胺杂化复合材料中。修饰后的电极被标记为 PTMSPA/HRP-GOx。在修饰电极上实现了 HRP 的直接电子转移。此外，该电极对过氧化氢的还原表现出优异的生物电化学催化活性。在 PTMSPA/HRP-GOx 修饰电极上的响应电流与葡萄糖浓度在 1 至 20mM 之间呈现出良好的线性关系，响应时间为 7s。因此，修饰电极通过各组分的协同影响表现出聚苯胺和硅网络的综合优势。PTMSPA 组装已显示出作为第三代安培生物传感器的潜力。
