College of Chemistry, Chemical Engineering and Materials Science, Engineering Research Center of Pesticide and Medicine Intermediate Clean Production, Ministry of Education, Key Laboratory of Molecular and Nano Probes, Shandong Normal University, Jinan 250014, People's Republic of China.
Biosens Bioelectron. 2010 Jun 15;25(10):2244-8. doi: 10.1016/j.bios.2010.03.006. Epub 2010 Mar 9.
A mediator-free hydrogen peroxide (H(2)O(2)) biosensor was fabricated based on immobilization of horseradish peroxidase (HRP) on layered calcium carbonate-gold nanoparticles (CaCO(3)-AuNPs) inorganic hybrid composite. The proposed biosensor showed a strong electrocatalytic activity toward the reduction of H(2)O(2), which could be attributed to the favored orientation of HRP in the well-confined surface as well as the high electrical conductivity of the resulting CaCO(3)-AuNPs inorganic hybrid composite. The hybrid composite was obtained by the adsorption of AuNPs onto the surfaces of layered CaCO(3) through electrostatic interaction. The key analytical parameters relative to the biosensor performance such as pH and applied potential were optimized. The developed biosensor also exhibited a fast amperometric response (3s), a good linear response toward H(2)O(2) over a wide range of concentration from 5.0x10(-7) to 5.2x10(-3)M, and a low detection limit of 1.0x10(-7)M. The facile, inexpensive and reliable sensing platform based on layered CaCO(3)-AuNPs inorganic hybrid composite should hold a huge potential for the fabrication of more other biosensors.
一种无介体的过氧化氢（H₂O₂）生物传感器是基于辣根过氧化物酶（HRP）固定在层状碳酸钙-金纳米粒子（CaCO₃-AuNPs）无机杂化复合材料上制备的。所提出的生物传感器对 H₂O₂的还原表现出很强的电催化活性，这可归因于 HRP 在良好受限表面上的有利取向以及所得 CaCO₃-AuNPs 无机杂化复合材料的高导电性。通过静电相互作用将 AuNPs 吸附到层状 CaCO₃的表面上，得到了杂化复合材料。对与生物传感器性能相关的关键分析参数，如 pH 值和应用电位进行了优化。所开发的生物传感器还表现出快速的电流响应（3 秒），对 H₂O₂在宽浓度范围内（从 5.0x10⁻⁷到 5.2x10⁻³M）具有良好的线性响应，检测限低至 1.0x10⁻⁷M。基于层状 CaCO₃-AuNPs 无机杂化复合材料的这种简便、廉价且可靠的传感平台，应该为制造更多其他生物传感器提供巨大的潜力。
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