Graduate School of Science, Osaka City University, Osaka 558-8585, Japan.
Biochem Biophys Res Commun. 2010 Jan 8;391(2):1251-4. doi: 10.1016/j.bbrc.2009.12.053. Epub 2009 Dec 16.
We investigated the fatty acid compositions of phospholipids in Drosophila melanogaster lines showing rapid (CR), intermediate (CTL), or slow (CS) recovery from chill coma, which were established by artificial selection or by free recombination without selection. Compared to CTL, CS showed a low composition of dienoic acids and a small number of double bonds in the fatty acids. The ratio of unsaturated fatty acids and saturated fatty acids (UFAs/SFAs) was significantly lower in CS than in CTL. CR had higher monoenoic acid composition and lower dienoic acid composition than CTL. In addition, the amount of SFAs was lower and therefore the UFAs/SFAs ratio considerably higher in CR than in CTL. These changes in phospholipid fatty acids probably contributed to losing and maintaining the homeoviscosity of the cellular membranes in CS and CR, respectively, at low temperature and therefore produced their distinct phenotypes in recovery from chill coma.
我们研究了表现出快速（CR）、中等（CTL）或缓慢（CS）从冷休克中恢复的黑腹果蝇系的磷脂脂肪酸组成，这些系是通过人工选择或无选择的自由重组建立的。与 CTL 相比，CS 表现出较低的二烯酸组成和较少的脂肪酸双键。CS 中的不饱和脂肪酸与饱和脂肪酸（UFAs/SFAs）的比例明显低于 CTL。CR 比 CTL 具有更高的单烯酸组成和更低的二烯酸组成。此外，CS 中的 SFAs 含量较低，因此 UFAs/SFAs 比率明显高于 CTL。这些磷脂脂肪酸的变化可能分别有助于 CS 和 CR 在低温下失去和维持细胞膜的同型粘滞性，并因此在从冷休克中恢复时表现出不同的表型。
