Sugenoya A, Mizuno E, Haniuda M, Fujimori M, Masuda H, Kasuga Y, Kobayashi S, Iida F
Department of Surgery, Shinshu University School of Medicine, Matsumoto, Japan.
Acta Endocrinol (Copenh). 1991 Jan;124(1):115-20. doi: 10.1530/acta.0.1240115.
The presence of thyroid hormone autoantibodies was investigated in a 48-year-old non-goitrous woman with high levels of serum total T3 and free T3. Her other thyroid function tests were normal. The presence of thyroid hormone autoantibodies were assessed in serum, acid-charcoal treated serum, IgG fractions and in a purified IgG fraction. the IgG fraction was separated from serum by a protein A-Sepharose CL-4B column chromatography and purified by gel filtration chromatography using Sephacryl S 200. Sera from normal individuals were used as controls. The results showed that the increased serum total and free T3 levels were caused by the presence of anti-T3 IgG autoantibodies. The results also indicate that protein A column chromatography is useful as a screening method for gross qualitative analyses of thyroid hormone autoantibodies.
对一名48岁无甲状腺肿且血清总T3和游离T3水平升高的女性进行了甲状腺激素自身抗体检测。她的其他甲状腺功能检查结果正常。在血清、酸炭处理血清、IgG组分以及纯化的IgG组分中评估甲状腺激素自身抗体的存在情况。通过蛋白A-琼脂糖CL-4B柱色谱从血清中分离出IgG组分，并使用Sephacryl S 200通过凝胶过滤色谱进行纯化。以正常个体的血清作为对照。结果显示，血清总T3和游离T3水平升高是由抗T3 IgG自身抗体的存在所致。结果还表明，蛋白A柱色谱作为甲状腺激素自身抗体总体定性分析的筛查方法很有用。
