Sakata S, Matsuda M, Ogawa T, Takuno H, Matsui I, Sarui H, Yasuda K
Third Department of Internal Medicine, Gifu University School of Medicine, Japan.
Clin Endocrinol (Oxf). 1994 Sep;41(3):365-70. doi: 10.1111/j.1365-2265.1994.tb02558.x.
The prevalence of thyroid hormone autoantibodies in patients with thyroid disorders has been well described, although the results have been variable. However, the prevalence of thyroid hormone autoantibodies in apparently healthy subjects remains unknown and its determination was the main objective of this study.
Sera obtained from 880 healthy subjects (365 men and 515 women) were examined for thyroid hormone autoantibodies by immune precipitation using radiolabelled thyroid hormones or thyroid hormone analogues.
Anti-triiodothyronine (T3) and anti-thyroxine (T4) antibodies were detected in none (0%) and in 3 (0.34%), respectively, of the 880 individuals studied using radiolabelled thyroid hormones. Similar tests in 385 healthy subjects using radiolabelled thyroid hormone analogues (polyaminocarboxy T3 and T4) showed the presence of anti-T3 and anti-T4 antibodies in 3 (0.78%) and in 4 (1.04%), respectively. None of the subjects had both anti-T3 and anti-T4 antibodies. Thus, the prevalence of anti-T3 or anti-T4 antibodies among healthy population was concluded to be as high as 1.8%. Radiolabelled thyroid hormone analogue binding to purified immunoglobulin G (IgG), with or without the addition of an excess of unlabelled thyroid hormones, confirmed specific binding of thyroid hormones to the thyroid hormone autoantibody-positive IgGs. The presence and class specificity of thyroid hormone autoantibodies was confirmed by the Ouchterlony immunodiffusion/autoradiography method, which showed biphenotypic heavy chain (IgG and IgA) and kappa (kappa) light chain specificities. Serum concentrations of free thyroid hormones and TSH in thyroid hormone autoantibody-positive sera were within the normal range.
These results indicate that the occurrence of thyroid hormone autoantibodies among the healthy Japanese population is fairly common. However, interference of thyroid hormone autoantibodies in the radioimmunoassay of free thyroid hormones is exceptional.
甲状腺疾病患者中甲状腺激素自身抗体的患病率已有充分描述，尽管结果存在差异。然而，健康人群中甲状腺激素自身抗体的患病率仍不清楚，本研究的主要目的就是确定这一患病率。
采用放射性标记的甲状腺激素或甲状腺激素类似物免疫沉淀法，检测了880名健康受试者（365名男性和515名女性）血清中的甲状腺激素自身抗体。
在使用放射性标记甲状腺激素检测的880名个体中，未检测到抗三碘甲状腺原氨酸（T3）抗体，抗甲状腺素（T4）抗体的检出率为3例（0.34%）。在385名健康受试者中使用放射性标记的甲状腺激素类似物（聚氨基羧基T3和T4）进行类似检测，结果显示抗T3和抗T4抗体的检出率分别为3例（0.78%）和4例（1.04%）。所有受试者均未同时存在抗T3和抗T4抗体。因此，健康人群中抗T3或抗T4抗体的患病率高达1.8%。放射性标记的甲状腺激素类似物与纯化的免疫球蛋白G（IgG）结合，无论是否添加过量未标记的甲状腺激素，均证实甲状腺激素与甲状腺激素自身抗体阳性的IgG存在特异性结合。甲状腺激素自身抗体的存在及类别特异性通过欧氏免疫扩散/放射自显影法得以证实，该方法显示出双表型重链（IgG和IgA）和κ（kappa）轻链特异性。甲状腺激素自身抗体阳性血清中游离甲状腺激素和促甲状腺激素（TSH）的血清浓度在正常范围内。
这些结果表明，健康日本人群中甲状腺激素自身抗体的出现相当普遍。然而，甲状腺激素自身抗体对游离甲状腺激素放射免疫测定的干扰情况较为罕见。
