Department of Dermatology, the General Hospital of the Air Force, Beijing 1000142, PR China.
Eur J Pharmacol. 2010 Mar 10;629(1-3):147-52. doi: 10.1016/j.ejphar.2009.12.007. Epub 2009 Dec 13.
Allergic contact dermatitis is a prototypic T-cell-mediated cutaneous inflammatory response. Multiple cell types, inflammatory mediators and cytokines are involved in the regulation of immunologic and inflammatory processes in allergic contact dermatitis. Aloperine is an isolated alkaloid found in the plant of Sophora alopecuroides L. It has been clinically proved effective in China for a long time for skin inflammatory diseases such as allergic contact dermatitis. However, the mechanism of aloperine on allergic contact dermatitis is largely unknown. Therefore, the aim of this study was to investigate the effect of aloperine on 2, 4-dinitrofluorobenzene (DNFB)-induced allergic contact dermatitis in BALB/c mice and the possible underlying mechanisms. The results showed that topical application of DNFB on the ear provoked typical allergic contact dermatitis with ear swelling and ear erythema in BALB/c mice. Treatments with 1% aloperine suppressed DNFB-induced increase in ear thickness and ear erythema. Moreover, 1% aloperine treatment significantly decreased the up-regulated mRNA and protein levels of tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta) and interleukin-6 (IL-6) induced by DNFB in ear biopsy homogenates. Our findings suggest that aloperine greatly improves the DNFB-induced allergic contact dermatitis in mice. The therapeutic mechanism might be related to the reduction of TNF-alpha, IL-1beta and IL-6 production induced by DNFB.
变应性接触性皮炎是一种典型的 T 细胞介导的皮肤炎症反应。多种细胞类型、炎症介质和细胞因子参与调节变应性接触性皮炎的免疫和炎症过程。阿萝柏碱是从苦参属植物苦参中分离得到的一种生物碱。在中国，阿萝柏碱长期以来在治疗变应性接触性皮炎等皮肤炎症性疾病方面已被临床证实有效。然而，阿萝柏碱治疗变应性接触性皮炎的机制在很大程度上尚不清楚。因此，本研究旨在探讨阿萝柏碱对二硝基氟苯（DNFB）诱导的 BALB/c 小鼠变应性接触性皮炎的作用及其可能的机制。结果表明，DNFB 涂于耳可引起 BALB/c 小鼠典型的变应性接触性皮炎，表现为耳肿胀和耳红斑。1%阿萝柏碱处理可抑制 DNFB 诱导的耳厚度增加和耳红斑。此外，1%阿萝柏碱处理可显著降低 DNFB 诱导的耳组织匀浆中肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）和白细胞介素-6（IL-6）mRNA 和蛋白水平的上调。我们的研究结果表明，阿萝柏碱可显著改善 DNFB 诱导的小鼠变应性接触性皮炎。其治疗机制可能与降低 DNFB 诱导的 TNF-α、IL-1β 和 IL-6 的产生有关。
