Institute of Medical Research, MMA, Crnotravska 17, 11002 Belgrade, Serbia.
J Biomater Appl. 2010 Sep;25(3):269-86. doi: 10.1177/0885328209354613. Epub 2009 Dec 11.
Cu-Al-Ni shape memory alloys (SMAs) have been investigated as materials for medical devices, but little is known about their biocompatibility. The aim of this work was to study the response of rat peritoneal macrophages (PMØ) to a Cu-Al-Ni SMA in vitro, by measuring the functional activity of mitochondria, necrosis, apoptosis, and production of proinflammatory cytokines. Rapidly solidified (RS) thin ribbons were used for the tests. The control alloy was a permanent mold casting of the same composition, but without the shape memory effect. Our results showed that the control alloy was severely cytotoxic, whereas RS ribbons induced neither necrosis nor apoptosis of PMØ. These findings correlated with the data that RS ribbons are significantly more resistant to corrosion compared to the control alloy, as judged by the lesser release of Cu and Ni in the conditioning medium. However, the ribbons generated intracellular reactive oxygen species and upregulated the production of IL-6 by PMØ. These effects were almost completely abolished by conditioning the RS ribbons for 5 weeks. In conclusion, RS significantly improves the corrosion stability and biocompatibility of Cu-Al-Ni SMA. The biocompatibility of this functional material could be additionally enhanced by conditioning the ribbons in cell culture medium.
铜-铝-镍形状记忆合金（SMA）已被研究作为医疗器械的材料，但对其生物相容性知之甚少。本工作旨在通过测量线粒体的功能活性、坏死、凋亡和促炎细胞因子的产生，研究大鼠腹腔巨噬细胞（PMØ）对体外铜-铝-镍 SMA 的反应。使用快速凝固（RS）薄带进行测试。对照合金是相同成分的永久模铸造，但没有形状记忆效应。我们的结果表明，对照合金具有严重的细胞毒性，而 RS 带既不会引起 PMØ 的坏死也不会引起凋亡。这些发现与以下数据相关，即与对照合金相比，RS 带具有更高的耐腐蚀性，因为在调理介质中释放的 Cu 和 Ni 较少。然而，这些带会产生细胞内活性氧，并上调 PMØ 中 IL-6 的产生。通过将 RS 带在细胞培养基中调理 5 周，这些作用几乎完全被消除。总之，RS 显著提高了铜-铝-镍 SMA 的耐腐蚀性和生物相容性。通过在细胞培养基中调理带，可以进一步提高这种功能材料的生物相容性。
