Harris David, Olechno Joe, Datwani Sammy, Ellson Richard
Labcyte Inc., Sunnyvale, CA 94089, USA.
J Biomol Screen. 2010 Jan;15(1):86-94. doi: 10.1177/1087057109351027. Epub 2009 Dec 11.
More accurate dose-response curves can be constructed by eliminating aqueous serial dilution of compounds. Traditional serial dilutions that use aqueous diluents can result in errors in dose-response values of up to 4 orders of magnitude for a significant percentage of a compound library. When DMSO is used as the diluent, the errors are reduced but not eliminated. The authors use acoustic drop ejection (ADE) to transfer different volumes of model library compounds, directly creating a concentration gradient series in the receiver assay plate. Sample losses and contamination associated with compound handling are therefore avoided or minimized, particularly in the case of less water-soluble compounds. ADE is particularly well suited for assay miniaturization, but gradient volume dispensing is not limited to miniaturized applications.
通过消除化合物的水溶液系列稀释，可以构建更准确的剂量反应曲线。使用水性稀释剂的传统系列稀释可能导致化合物库中很大比例的化合物剂量反应值误差高达4个数量级。当使用二甲亚砜作为稀释剂时，误差会减小但不会消除。作者使用声学液滴喷射（ADE）来转移不同体积的模型库化合物，直接在接收测定板中创建浓度梯度系列。因此，避免或最小化了与化合物处理相关的样品损失和污染，特别是对于水溶性较差的化合物。ADE特别适合于测定小型化，但梯度体积分配不限于小型化应用。
