Materials Research and Education Center, Department of Mechanical Engineering, Auburn University, Auburn, Alabama 36849, United States.
Anal Chem. 2011 Aug 15;83(16):6148-53. doi: 10.1021/ac201177g. Epub 2011 Jul 19.
We demonstrate the accommodation of log-scale concentration gradients of inhibitors on a single microfluidic chip with a semidirect dilution capability of reagents for the determination of the half-inhibitory concentration or IC(50). The chip provides a unique tool for hosting a wide-range of concentration gradient for studies that require an equal distribution of measuring points on a logarithmic scale. Using Matrix metalloproteinase IX and three of its inhibitors, marimastat, batimastat, and CP471474, we evaluated the IC(50) of each inhibitor with a single experiment. The present work could be applied to the systematic study of biochemical binding and inhibition processes particularly in the field of mechanistic enzymology and the pharmaceutical industry.
我们在单个微流控芯片上演示了抑制剂的对数浓度梯度的适应，该芯片具有半直接试剂稀释能力，用于测定半抑制浓度或 IC（50）。该芯片为 hosted 提供了一种独特的工具，用于在需要在对数尺度上均匀分布测量点的研究中，提供大范围的浓度梯度。我们使用基质金属蛋白酶 9 及其三种抑制剂，即 marimastat、batimastat 和 CP471474，在单个实验中评估了每种抑制剂的 IC（50）。本工作可应用于生化结合和抑制过程的系统研究，特别是在机制酶学和制药工业领域。
