Graduate School of Materials Science, Nara Institute of Science and Technology, Ikoma, Nara, Japan.
Nanotechnology. 2010 Jan 29;21(4):045305. doi: 10.1088/0957-4484/21/4/045305. Epub 2009 Dec 16.
Newly designed porter proteins, which catch gold nanoparticles and deliver the nanoparticles selectively to a silicon dioxide (SiO(2)) surface under the specific conditions were reported. Recombinant apoferritin subunits, each of which has gold-binding peptide and titanium-binding peptide at the C- and N-terminus, respectively, can efficiently encapsulate a gold nanoparticle. The bio-conjugate, a nanogold and surrounding mutant protein subunits, had a property which can deliver itself to the SiO(2) surface through the interaction. In theory, our genetically manipulated apoferritin subunits can encapsulate gold nanoparticles of various sizes, which is a promising property for applications involving surface plasmon resonance.
报道了一种新设计的载体蛋白，它可以捕获金纳米粒子，并在特定条件下将纳米粒子有选择性地递送到二氧化硅（SiO(2)）表面。重组脱铁铁蛋白亚基，每个亚基在 C 端和 N 端分别具有金结合肽和钛结合肽，可以有效地包裹金纳米粒子。生物缀合物，即纳米金和周围的突变蛋白亚基，具有通过相互作用将自身递送到 SiO(2)表面的性质。从理论上讲，我们经过基因改造的脱铁铁蛋白亚基可以包裹各种尺寸的金纳米粒子，这对于涉及表面等离子体共振的应用来说是一个很有前途的特性。
