用于卵清蛋白检测中信号增强的滚环扩增

Rolling circle amplification for signal enhancement in ovalbumin detection.

作者信息

Kobori Toshiro, Matsumoto Atsuko, Takahashi Hirokazu, Sugiyama Shigeru

机构信息

Nanobiotechnology Laboratory, National Food Research Institute, National Agriculture and Food Research Organization, 2-1-12 Kan-nondai, Tsukuba 305-9642, Japan.

出版信息

Anal Sci. 2009 Dec;25(12):1381-3. doi: 10.2116/analsci.25.1381.

DOI:10.2116/analsci.25.1381

PMID:20009321

Abstract

We have developed a rolling circle amplification-procedure as a method to enhance signals of immunoassay (immuno-RCA) to detect one of the major food allergens, ovalbumin (OVA). Immuno-RCA in combination with a fluorescent dye and a circular DNA probe prepared by intramolecular ligation easily allowed the real-time detection of OVA, and could apparently detect signals from OVA with concentrations ranging from 10(-12) to 10(-7) g/mL. Therefore, sensitive and easily handled aspects of this method would contribute to an effective signal enhancement in immunoassay for food allergen detections.

摘要

我们开发了一种滚环扩增程序作为增强免疫分析信号（免疫滚环扩增）的方法，用于检测主要食物过敏原之一的卵清蛋白（OVA）。免疫滚环扩增与荧光染料和通过分子内连接制备的环状DNA探针相结合，能够轻松实现对OVA的实时检测，并且显然可以检测浓度范围为10（-12）至10（-7）g/mL的OVA信号。因此，该方法的灵敏性和易操作性将有助于在食物过敏原检测的免疫分析中实现有效的信号增强。