Department of Obstetrics and Gynecology, School of Medicine, Kitasato University, Sagamihara, Kanagawa, Japan.
Int J Gynecol Cancer. 2009 Nov;19(8):1396-401. doi: 10.1111/IGC.0b013e3181b661a4.
The aim of this study was to clarify the relation of human papillomavirus (HPV) genotypes and physical status in the cervical neoplasm of Japanese patients with the grade of the disease. Human papillomavirus genotype was detected using a linear array genotyping assay. Human papillomavirus status, diffuse or punctate signal pattern, was studied by biotynyl-tyramide-based in situ hybridization for positive cases of HPV-16. Human papillomavirus types 16, 52, 58, and 31, in descending order of frequency, were prevalent. The rates of HPV infection in patients with cervical intraepithelial neoplasia (CIN) or squamous cell carcinoma (SCC) were significantly higher than those in patients without cervical lesions. The frequency of HPV single infection in SCC was higher than that in CIN1 or CIN2. In an unspecified-risk HPV, types 66 and 70 were found in SCC and 62, 71, and 82 were detected in CIN3. The diffuse pattern was more frequent in CIN, and the punctate pattern was more frequent in SCC. Human papillomavirus types 16, 52, 58, and 31 were frequently detected in Japanese women with cervical neoplasias, and several unspecified-risk HPVs might be high-risk types. A single infection of HPV and a punctate signal pattern seemed to be closely correlated with cervical carcinogenesis.
本研究旨在阐明人乳头瘤病毒（HPV）基因型与日本宫颈肿瘤患者疾病分级之间的关系。采用线性阵列基因分型检测 HPV 基因型。通过针对 HPV-16 阳性病例的生物素-酪酰胺原位杂交研究 HPV 状态（弥漫或点状信号模式）。HPV 类型 16、52、58 和 31 依次为常见类型。宫颈上皮内瘤变（CIN）或鳞状细胞癌（SCC）患者的 HPV 感染率显著高于无宫颈病变患者。SCC 患者 HPV 单一感染率高于 CIN1 或 CIN2。在未指定风险 HPV 中，66 和 70 型见于 SCC，62、71 和 82 型见于 CIN3。弥漫模式在 CIN 中更为常见，点状模式在 SCC 中更为常见。HPV 类型 16、52、58 和 31 经常在日本患有宫颈肿瘤的女性中检测到，几种未指定风险 HPV 可能是高危型。HPV 单一感染和点状信号模式似乎与宫颈癌变密切相关。
