人乳头瘤病毒（HPV）基因分型和 HPV16 变异分析在宫颈腺性和鳞状肿瘤病变中的应用。
HPV genotyping and HPV16 variant analysis in glandular and squamous neoplastic lesions of the uterine cervix.
机构信息
DDL Diagnostic Laboratory, Voorburg, The Netherlands.
出版信息
Gynecol Oncol. 2010 May;117(2):297-301. doi: 10.1016/j.ygyno.2010.02.003. Epub 2010 Mar 6.
OBJECTIVE
The objective of the study was to compare the distribution of HPV genotypes and HPV16 variants in glandular and squamous cervical neoplasia.
METHODS
Cases of endocervical adenocarcinoma in-situ (AIS, n=33) invasive adenocarcinoma (ADCA, n=55), cervical intraepithelial neoplasia-3 (CIN3, n=130) and squamous cell carcinoma (SCC, n=60) were collected at the New York Hospital and tested for HPV using SPF(10)PCR-LIPA(25) (version 1) assays and for HPV16 variants using a multiplex PCR and reverse hybridization assay.
RESULTS
There was a difference between the spectrum of HPV genotypes detected in glandular and squamous neoplasia: 13 different HPV genotypes were detected in CIN3 as single infections and 11 in SCC, while only 4 single genotypes were detected in AIS and 3 in ADCA. The most common single HPV types in CIN3 were HPV16, 31, and 52 (56.9%, 10%, 8.4%, respectively). In SCC the most common were HPV16, 18 and 31 (70%, 6.5%, 4.9%). In AIS, HPV16, 18, 45 and 35 accounted for 69.7%, 27.2%, 3%, 3% of cases. The three single types in ADCA were HPV16 (43.6%), HPV18 (41.8%) and HPV45 (10.9%). European variants of HPV16 were the most common in CIN3 (83.8%), SCC (71.4%) and AIS (73.9%). In ADCA the Asian American (AA) variant was the most common (41.7%) followed by European variants (33.3%). AA variant was also detected in 17.4%, 4.1%, and 2.4% of HPV16 positive AIS, CIN3 and SCC, respectively.
CONCLUSION
Asian American variant of HPV16, HPV18 and HPV45 are preferentially associated with cervical adenocarcinoma as compared to squamous cell carcinoma.
目的
本研究旨在比较 HPV 基因型在宫颈腺性和鳞状肿瘤中的分布，并分析 HPV16 变体的分布情况。
方法
收集纽约医院的宫颈原位腺癌（AIS）、浸润性腺癌（ADCA）、宫颈上皮内瘤变 3 级（CIN3）和宫颈鳞状细胞癌（SCC）标本各 33、55、130 和 60 例，采用 SPF(10)PCR-LIPA(25)（版本 1）检测 HPV，采用多重 PCR 和反向杂交检测 HPV16 变体。
结果
在腺性和鳞状肿瘤中，HPV 基因型的分布存在差异：在 CIN3 中，单一感染的 HPV 基因型有 13 种，在 SCC 中则有 11 种，而在 AIS 中只有 4 种单一基因型，ADCA 中只有 3 种。在 CIN3 中，最常见的单一 HPV 类型是 HPV16、31 和 52（分别占 56.9%、10%、8.4%）。在 SCC 中，最常见的是 HPV16、18 和 31（分别占 70%、6.5%、4.9%）。在 AIS 中，HPV16、18、45 和 35 分别占 69.7%、27.2%、3%、3%。在 ADCA 中，三种单一 HPV 类型分别为 HPV16（43.6%）、HPV18（41.8%）和 HPV45（10.9%）。欧洲 HPV16 变体在 CIN3（83.8%）、SCC（71.4%）和 AIS（73.9%）中最为常见。在 ADCA 中，亚洲裔美国人（AA）变体最为常见（41.7%），其次是欧洲变体（33.3%）。在 HPV16 阳性的 AIS、CIN3 和 SCC 中，分别有 17.4%、4.1%和 2.4%检测到 AA 变体。
结论
与宫颈鳞状细胞癌相比，HPV16、18 和 45 的亚洲裔美国人变体更倾向于与宫颈腺癌相关。
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