小鼠胶原诱导性关节炎

Collagen-induced arthritis in mice.

作者信息

Bevaart Lisette, Vervoordeldonk Margriet J, Tak Paul P

机构信息

Division of Clinical Immunology and Rheumatology, Academic Medical Center/University of Amsterdam, Amsterdam, The Netherlands.

出版信息

Methods Mol Biol. 2010;602:181-92. doi: 10.1007/978-1-60761-058-8_11.

DOI:10.1007/978-1-60761-058-8_11

PMID:20012399

Abstract

Collagen-induced arthritis (CIA) in mice is an animal model for rheumatoid arthritis (RA) and can be induced in DBA/1 and C57BL/6 mice using different protocols. The CIA model can be used to unravel mechanisms involved in the development of arthritis and is frequently used to study the effect of new therapeutics. The development of a CIA model in C57BL/6 mice recently enabled researchers to use knockout mice on this background for arthritis research.In this chapter, the protocol for induction of arthritis in both mice strains is described, including the monitoring of clinical arthritis and paw swelling in the mice during the experiment. Furthermore, protocols for decalcification of paws and for the detection of collagen-specific antibodies in mice sera are described.

摘要

小鼠胶原诱导性关节炎（CIA）是类风湿性关节炎（RA）的动物模型，可通过不同方案在DBA/1和C57BL/6小鼠中诱导产生。CIA模型可用于揭示关节炎发病机制，常用于研究新疗法的效果。最近，C57BL/6小鼠CIA模型的建立使研究人员能够在此背景下使用基因敲除小鼠进行关节炎研究。本章描述了两种小鼠品系关节炎诱导方案，包括实验过程中小鼠临床关节炎和爪肿胀的监测。此外，还描述了爪脱钙和小鼠血清中胶原特异性抗体检测的方案。