bol-miRNA172a对FAN表达的影响解释白菜叶的抗炎作用
The Anti-Inflammatory Effect of Cabbage Leaves Explained by the Influence of bol-miRNA172a on FAN Expression.
作者信息
Kasarello Kaja, Köhling Iwona, Kosowska Anna, Pucia Katarzyna, Lukasik Anna, Cudnoch-Jedrzejewska Agnieszka, Paczek Leszek, Zielenkiewicz Urszula, Zielenkiewicz Piotr
机构信息
Centre for Preclinical Research, Department of Experimental and Clinical Physiology, Medical University of Warsaw, Warsaw, Poland.
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland.
出版信息
Front Pharmacol. 2022 Mar 24;13:846830. doi: 10.3389/fphar.2022.846830. eCollection 2022.
Recently, the possibility of cross-kingdom gene expression regulation by miRNAs from other species ("xenomiRs"), specifically from plants, has acquired scientific meaning. Based on the one of oldest methods for dealing with inflammation the use of cabbage leaf compresses, we investigated the effects of derived miR172a on the potential human target gene encoding FAN (Factor Associated with Neutral Sphingomyelinase Activation) protein. experiments showed a decrease in FAN protein levels in both human and mouse cells transfected with bol-miRNA172a. As the FAN protein mediates inflammatory responses, the potential of miR172a to mitigate the inflammatory process was tested in a mouse model of rheumatoid arthritis. Animal studies showed the decreased oedema of inflamed paws in mouse with rheumatoid arthritis model induced after treatment with miR172a.
最近，来自其他物种（“异种miRNA”），特别是来自植物的miRNA进行跨物种基因表达调控的可能性已具有科学意义。基于最古老的治疗炎症的方法之一——使用卷心菜叶敷贴，我们研究了植物来源的miR172a对编码FAN（与中性鞘磷脂酶激活相关因子）蛋白的潜在人类靶基因的影响。实验表明，用bol-miRNA172a转染的人和小鼠细胞中FAN蛋白水平均降低。由于FAN蛋白介导炎症反应，因此在类风湿性关节炎小鼠模型中测试了miR172a减轻炎症过程的潜力。动物研究表明，用miR172a治疗后诱导的类风湿性关节炎模型小鼠的炎症爪子水肿减轻。
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