Díaz-González Julia, Domínguez Ana, Albornoz Jesús
Area de Genética, Departamento de Biología Funcional, Universidad de Oviedo, 33071, Oviedo, Spain.
Genetica. 2010 Jun;138(6):579-86. doi: 10.1007/s10709-009-9430-7. Epub 2009 Dec 11.
The intragenomic distribution of five retrotransposon families (297, 1731, copia, mdg1 and roo) in the species of the melanogaster complex was studied by comparing results of the Southern blotting technique in males and females with those of in situ hybridization. The degree of structural polymorphism of each family in the different species was also investigated by restriction enzyme analysis. It was found that genomic distribution is a trait that depends on the family and species. The distribution of roo is mainly euchromatic in the four species and 1731 is heterochromatic, but the distribution of families 297, copia and mdg1 is markedly different in the melanogaster and simulans clades. These families were mainly euchromatic in D. melanogaster but heterochromatic in its sibling species. In the simulans clade most copia and mdg1 elements are located on chromosome Y. Differences in genomic distribution are unrelated with structural conservation. The relation of intragenomic distribution to phylogeny, transpositional activity and the role of the host genome are discussed.
通过比较雄性和雌性的Southern印迹技术结果与原位杂交结果，研究了五个逆转座子家族（297、1731、copia、mdg1和roo）在黑腹果蝇复合体物种中的基因组内分布。还通过限制性酶切分析研究了每个家族在不同物种中的结构多态性程度。发现基因组分布是一个取决于家族和物种的特征。roo在四个物种中的分布主要在常染色质区，1731在异染色质区，但297、copia和mdg1家族在黑腹果蝇和拟果蝇分支中的分布明显不同。这些家族在黑腹果蝇中主要在常染色质区，但在其姐妹物种中在异染色质区。在拟果蝇分支中，大多数copia和mdg1元件位于Y染色体上。基因组分布的差异与结构保守性无关。讨论了基因组内分布与系统发育、转座活性以及宿主基因组作用的关系。
