Terrinoni A, Franco C D, Dimitri P, Junakovic N
Centro per lo Studio degli Acidi Nucleici C.N.R. Dipartimento di Genetica e Biologia Molecolare, Università La Sapienza, P. le A. Moro 5, 00185 Roma, Italy.
J Mol Evol. 1997 Aug;45(2):145-53. doi: 10.1007/pl00006214.
The intragenomic location of the elements of the I, G, jockey, F, and Doc transposon families has been studied by the Southern blot analysis, in 12 laboratory Drosophila melanogaster stocks. Elements located in euchromatin, heterochromatin, and on the Y chromosome are identified, and their stability has been assessed by comparing the autoradiographs detected in different stocks and analysis of individual flies. Evidence is shown suggesting that preferential location in euchromatin or heterochromatin and the distribution within heterochromatin are distinctive of transposon families. Elements located in heterochromatin can be unstable. These results are discussed in the context of the relationship between transposable elements and the host genome.
通过Southern印迹分析，在12个实验室黑腹果蝇品系中研究了I、G、jockey、F和Doc转座子家族元件的基因组内定位。鉴定了位于常染色质、异染色质和Y染色体上的元件，并通过比较不同品系中检测到的放射自显影片和对单个果蝇的分析来评估它们的稳定性。结果表明，转座子家族在常染色质或异染色质中的优先定位以及在异染色质内的分布具有独特性。位于异染色质中的元件可能不稳定。本文在转座元件与宿主基因组关系的背景下对这些结果进行了讨论。
