Drummond D R, Hennessey E S, Sparrow J C
Department of Biology, University of York, Heslington, U.K.
Biochem J. 1991 Feb 15;274 ( Pt 1)(Pt 1):301-3. doi: 10.1042/bj2740301.
Mutants of the Drosophila Act88F actin gene were transcribed and translated in vitro and their relative stabilities were examined using urea gradient gel electrophoresis. Most of the mutant actins (E334K, E364K, G366D, G368E and R372H) were as stable as the wild-type. V339I had a slight decrease in stability, and E316K was the least stable. The causes of the differences are discussed and contrasted with the behaviour of the mutants in vivo, where E316K has normal stability and V339I is the least stable.
对果蝇肌动蛋白基因Act88F的突变体进行体外转录和翻译，并使用尿素梯度凝胶电泳检测其相对稳定性。大多数突变型肌动蛋白（E334K、E364K、G366D、G368E和R372H）与野生型一样稳定。V339I的稳定性略有下降，而E316K最不稳定。文中讨论了差异产生的原因，并与这些突变体在体内的表现进行了对比，在体内E316K具有正常稳定性，而V339I最不稳定。
