Rogers S W, Rechsteiner M C
Prog Clin Biol Res. 1985;180:405-16.
The degradation rates of 35 proteins for which primary and X-ray structural data are available have been measured following their microinjection into HeLa cells. Each protein was radiolabeled by at least two techniques, and its degradation was measured in the presence or absence of the lysosomotropic agents, ammonia and chloroquine. The intracellular location of each protein was also determined by fractionation of injected cells using differential centrifugation in sucrose or by extraction in buffers containing Triton X-100. Preliminary analysis of these data indicates the following: lysosomes play a minor, nonselective role in the degradation of most microinjected proteins; the location of an injected protein within cells may significantly influence the rate at which it is degraded; and initial analyses of protein structural data such as molecular weight, isoelectric point, alpha or beta content, hydrophobicity, etc. have not yet revealed any strong correlations with stability.
在将35种有一级结构和X射线结构数据的蛋白质显微注射到HeLa细胞后，测定了它们的降解速率。每种蛋白质至少用两种技术进行放射性标记，并在存在或不存在溶酶体促效剂氨和氯喹的情况下测量其降解情况。还通过在蔗糖中进行差速离心对注射细胞进行分级分离，或在含有 Triton X - 100的缓冲液中进行提取，来确定每种蛋白质在细胞内的位置。对这些数据的初步分析表明：溶酶体在大多数显微注射蛋白质的降解中起次要的、非选择性的作用；注射的蛋白质在细胞内的位置可能会显著影响其降解速率；并且对蛋白质结构数据如分子量、等电点、α或β含量、疏水性等的初步分析尚未揭示与稳定性有任何强相关性。
