男性不育症中的精子 DNA 损伤：病因、检测方法和结果。
Sperm DNA damage in male infertility: etiologies, assays, and outcomes.
机构信息
Department of Urology, University of Michigan, Ann Arbor, MI 48109-0617, USA.
出版信息
J Assist Reprod Genet. 2010 Jan;27(1):3-12. doi: 10.1007/s10815-009-9359-x. Epub 2009 Dec 12.
Abstract
Male factor infertility is the sole cause of infertility in approximately 20% of infertile couples, with an additional 30% to 40% secondary to both male and female factors. Current means of evaluation of male factor infertility remains routine semen analysis including seminal volume, pH, sperm concentration, motility, and morphology. However, approximately 15% of patients with male factor infertility have a normal semen analysis and a definitive diagnosis of male infertility often cannot be made as a result of routine semen analysis. Attention has focused on the role of sperm nuclear DNA integrity in male factor infertility. Here we review the structure of human sperm chromatin, the etiology and mechanisms of sperm DNA damage, current tests available to assess sperm DNA integrity, and effect of sperm DNA integrity on reproductive outcomes.
摘要
男性因素不孕是约 20%不孕夫妇不孕的唯一原因，另有 30%至 40%的不孕原因同时涉及男性和女性因素。目前评估男性因素不孕的方法仍然是常规精液分析，包括精液量、pH 值、精子浓度、活力和形态。然而，约 15%的男性因素不孕患者的精液分析正常，因此常规精液分析往往无法明确男性不育症的诊断。人们关注精子核 DNA 完整性在男性因素不孕中的作用。本文综述了人类精子染色质的结构、精子 DNA 损伤的病因和机制、目前用于评估精子 DNA 完整性的检测方法，以及精子 DNA 完整性对生殖结局的影响。
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