“精子DNA完整性”随精液参数的波动及其与不孕症的关系。
Fluctuation of "sperm DNA integrity" in accordance with semen parameters, and it's relationship with infertility.
作者信息
Durmaz Ayşen, Dikmen Nurten, Gündüz Cumhur, Göker Ege Nazan Tavmergen, Tavmergen Erol
机构信息
Faculty of Medicine, Family Planning-Infertility Research and Treatment Center, Ege University, Bornova, Izmir, Turkey,
出版信息
J Assist Reprod Genet. 2014 Dec;31(12):1665-71. doi: 10.1007/s10815-014-0364-3. Epub 2014 Oct 25.
PURPOSE
Nowadays sperm analysis is used routinely to assess infertile men, but it's predictive value is still restricted. For this reason, new markers are needed for diagnosis and guide treatment. A DNA damage evaluation method, which is easily applicable for routine analysis is described in this paper to provide an important support in clinical evaluation.
METHODS
In this study, DNA damage evaluation was planned in infertile males with the COMET assay, a method which is simple, cheap, sensitive and reliable. For this purpose, the sperm DNA damage of normospermic, oligospermic, asthenospermic and teratospermic cases were investigated and the relationship between sperm parameters (sperm count, motility, morphology) and DNA damage was assessed.
RESULTS AND CONCLUSION
According to our results we suggest that the factors to blame for sperm DNA damage and semen quality are the same. Also it was found that the infertile males possess substantially more sperm DNA damage than fertile men do. Improving and using the DNA damage evaluation tests seem to improve the results in ART clinics.
目的
如今，精子分析常用于评估不育男性，但其预测价值仍有限。因此，需要新的标志物用于诊断和指导治疗。本文描述了一种易于应用于常规分析的DNA损伤评估方法，为临床评估提供重要支持。
方法
在本研究中，计划采用彗星试验对不育男性进行DNA损伤评估，该方法简单、廉价、灵敏且可靠。为此，研究了正常精子症、少精子症、弱精子症和畸形精子症患者的精子DNA损伤情况，并评估了精子参数（精子计数、活力、形态）与DNA损伤之间的关系。
结果与结论
根据我们的结果，我们认为导致精子DNA损伤和精液质量问题的因素相同。还发现不育男性的精子DNA损伤明显多于生育男性。改进并使用DNA损伤评估测试似乎能改善辅助生殖技术诊所的治疗效果。
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