Toronto Centre for Advanced Reproductive Technology, Toronto, Ontario, Canada; Department of Obstetrics and Gynecology, University of Toronto, Toronto, Ontario, Canada.
Division of Urology, Department of Surgery, Royal Victoria Hospital, McGill University Health Center, Montreal, Quebec, Canada.
Fertil Steril. 2014 Jun;101(6):1599-603. doi: 10.1016/j.fertnstert.2014.02.045. Epub 2014 Mar 26.
To investigate the association between seminal hyperviscosity, the extent of semenogelin degradation, and sperm DNA integrity (DNA fragmentation index [DFI] and high DNA stainability [HDS]) in semen from infertile couples.
Prospective study.
University-affiliated fertility center.
PATIENT(S): Twenty-four consecutive infertile couples with moderate or high seminal viscosity (hyperviscosity group) and 25 consecutive infertile couples with normal semen viscosity (control group) undergoing standard IVF.
INTERVENTION(S): Semen volume and seminal hyperviscosity, sperm concentration, motility, and morphology, level of semenogelin degradation (by immunoblotting), and sperm chromatin damage (by sperm chromatin structure assay and expressed as %DFI and %HDS) were evaluated.
MAIN OUTCOME MEASURES(S): Sperm %DFI and %HDS in the hyperviscosity group and the control group and the relationship between the extent of semenogelin degradation and seminal viscosity.
RESULT(S): Semen volume in couples with moderate and high seminal viscosity was significantly lower as compared with the control group. In addition, total motility and normal morphology were significantly lower in the couples with high seminal viscosity as compared with the control group; however, there were no significant differences in sperm %DFI and %HDS between the hyperviscosity group and the control group. In addition, there was no relationship between the extent of semenogelin degradation and seminal viscosity.
CONCLUSION(S): Our data suggest that seminal hyperviscosity (a posttesticular factor) is not an important cause of sperm DNA damage. Moreover, seminal hyperviscosity is not related to the degree of semenogelin degradation.
研究精液高黏滞度与精液中顶体蛋白酶降解程度和精子 DNA 完整性（DNA 碎片指数[DFI]和高 DNA 着色率[HDS]）之间的关系。
前瞻性研究。
大学附属生育中心。
24 对连续的中重度精液高黏滞度（高黏滞组）和 25 对连续的精液正常黏滞度（对照组）的不育夫妇，进行标准的 IVF。
评估精液量和精液高黏滞度、精子浓度、活力和形态、精液顶体蛋白酶降解水平（免疫印迹）和精子染色质损伤（精子染色质结构试验，以 DFI 和 HDS 的百分比表示）。
高黏滞组和对照组的精子 DFI 和 HDS 百分比以及精液顶体蛋白酶降解程度与精液黏度之间的关系。
中重度精液高黏滞度夫妇的精液量明显低于对照组。此外，高黏度组的总活力和正常形态明显低于对照组，但高黏度组和对照组之间的精子 DFI 和 HDS 百分比无显著差异。此外，精液顶体蛋白酶降解程度与精液黏度之间没有关系。
我们的数据表明，精液高黏滞度（睾丸后因素）不是精子 DNA 损伤的重要原因。此外，精液高黏滞度与精液顶体蛋白酶降解程度无关。
