大鼠体内外受精技术。
Techniques for in vitro and in vivo fertilization in the rat.
作者信息
Kashiwazaki Naomi, Seita Yasunari, Takizawa Akiko, Maedomari Naoki, Ito Junya, Serikawa Tadao
机构信息
Laboratory of Animal Reproduction, School of Veterinary Medicine, Azabu University, Kanagawa, Japan.
出版信息
Methods Mol Biol. 2010;597:311-22. doi: 10.1007/978-1-60327-389-3_22.
Although in vitro and in vivo fertilization are powerful tools for restoring conserved sperm as well as stocked males in the rat, the techniques have progressively gained importance. However, the techniques are not used extensively for efficient production of rat offspring, because the techniques require a great deal of skill. This chapter describes the protocols for in vitro and in vivo fertilization in the rat. Namely, sperm collection, sperm cryopreservation, pre-incubation of sperm, and insemination (co-culture with sperm and oocytes) for in vitro fertilization and intrauterine insemination for in vivo fertilization with fresh or frozen/thawed spermatozoa are provided.
尽管体外受精和体内受精是恢复大鼠中保存的精子以及储备雄性的有力工具，但这些技术的重要性与日俱增。然而，这些技术并未广泛用于高效生产大鼠后代，因为它们需要很高的技巧。本章描述了大鼠体外受精和体内受精的方案。具体而言，提供了用于体外受精的精子采集、精子冷冻保存、精子预孵育和授精（精子与卵母细胞共培养），以及用于体内受精的新鲜或冷冻/解冻精子的子宫内授精。
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