School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai, People's Republic of China.
Planta Med. 2010 May;76(8):803-8. doi: 10.1055/s-0029-1240689. Epub 2009 Dec 14.
From the dried roots of Campylotropis hirtella (Franch.) Schindl., five novel isoflavonoids, 3( S)-7,2',4'-trihydroxy-5,5'-dimethoxy-6-(3-methylbut-2-enyl)-isoflavan ( 1), 3( S)-2',4'-dihydroxy-5,5'-dimethoxy-(6'',6''-dimethylpyrano)-(2'',3'':7,6)-isoflavan ( 2), 3( R)-5,4'-dihydroxy-2'-methoxy-3'-(3-methylbut-2-enyl)-(6'',6''-dimethylpyrano)-(7,6 : 2'',3'')-isoflavanone ( 3), 3( R)-5,4'-dihydroxy-2'-methoxy-(6'',6''-dimethylpyrano)-(7,6: 2'',3'')-isoflavanone ( 4), and 3( R)-5,4'-dihydroxy-7,2'-dimethoxy-6-geranylisoflavanone ( 5) were isolated. The structures of the compounds were elucidated on the basis of spectroscopic analysis. All isolates exhibited good immunosuppressive activities on mitogen-induced splenocyte proliferation, and their cytotoxicity on splenic lymphocytes was also tested.
从山黧豆（Campylotropis hirtella（Franch.）Schindl.）的干燥根部分离得到五种新的异黄酮类化合物，3( S)-7,2',4'-三羟基-5,5'-二甲氧基-6-(3-甲基-2-丁烯基)-异黄酮（1）、3( S)-2',4'-二羟基-5,5'-二甲氧基-（6'',6''-二甲基吡喃基）-（2'',3'':7,6）-异黄酮（2）、3( R)-5,4'-二羟基-2'-甲氧基-3'-(3-甲基-2-丁烯基)-(6'',6''-二甲基吡喃基）-（7,6：2'',3'）-异黄酮酮（3）、3( R)-5,4'-二羟基-2'-甲氧基-（6'',6''-二甲基吡喃基）-（7,6：2'',3'）-异黄酮酮（4）和 3( R)-5,4'-二羟基-7,2'-二甲氧基-6-香叶基异黄酮酮（5）。基于光谱分析确定了化合物的结构。所有分离物均表现出良好的免疫抑制活性，能够抑制有丝分裂原诱导的脾细胞增殖，同时还测试了它们对脾淋巴细胞的细胞毒性。
