Chen H T, Chiou C S, Chang W C
Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan, China.
Biochim Biophys Acta. 1991 Feb 16;1088(2):315-8. doi: 10.1016/0167-4781(91)90071-s.
A carp genomic DNA clone containing the carp prolactin (Prl) gene was isolated with carp Prl cDNA as a probe. The organization of the carp Prl gene was determined by restriction nuclease mapping and nucleotide sequencing. The Prl gene comprises approx. 2.8 kilobasepairs (kb) of DNA including the 5'-flanking region, five exons, four introns and the 3'-flanking region. Analysis of the 5'-flanking region reveals (1) the sequence TATATAAT at positions -38 to -31 upstream from the cap site which was found to be a guanine residue, and (2) the palindrome, CTCATTGCATATACAAATGAG at positions -79 to -59. The carp Prl gene matches with the reported cDNA except for one difference in coding region and five in the 3'-flanking region, while the encoded amino acid sequences are identical. The arrangement of exons and introns is very similar to that seen in carp GH as well as mammalian Prl, which, however, have much longer introns.
以鲤鱼催乳素（Prl）cDNA为探针，分离出了一个包含鲤鱼Prl基因的鲤鱼基因组DNA克隆。通过限制性核酸酶图谱分析和核苷酸测序确定了鲤鱼Prl基因的结构。Prl基因由约2.8千碱基对（kb）的DNA组成，包括5'侧翼区、五个外显子、四个内含子和3'侧翼区。对5'侧翼区的分析显示：（1）在转录起始位点上游-38至-31位处有序列TATATAAT，该位点被发现是一个鸟嘌呤残基；（2）在-79至-59位处有回文序列CTCATTGCATATACAAATGAG。除了编码区有一处差异和3'侧翼区有五处差异外，鲤鱼Prl基因与已报道的cDNA相匹配，但其编码的氨基酸序列是相同的。外显子和内含子的排列与鲤鱼生长激素（GH）以及哺乳动物Prl中的排列非常相似，不过哺乳动物Prl的内含子要长得多。
