鲢鱼和鳙鱼催乳素的分子克隆

Molecular cloning of silver carp and bighead carp prolactin.

作者信息

Chang Y S, Huang F L, Lo T B

机构信息

Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan.

出版信息

Gen Comp Endocrinol. 1992 Aug;87(2):260-5. doi: 10.1016/0016-6480(92)90030-n.

DOI:10.1016/0016-6480(92)90030-n

PMID:1398019

Abstract

The cDNAs encoding the prolactin of silver carp (scPRL) and bighead carp (bcPRL) have been cloned. Deduced from the nucleotide sequences, both scPRL and bcPRL are composed of 187 amino acid residues. Only one residue is different between scPRL and bcPRL. Homology analysis indicates that scPRL and bcPRL are highly homologous to carp PRL (97%), relatively conserved in relation to PRLs of salmon, trout, and tilapia (64-69%), and diversified from avian and mammalian PRL (30-35%). Similar to PRLs of other species of fish, scPRL and bcPRL lack the first 12 N-terminal residues of avian and mammalian PRLs.

摘要

编码鲢鱼催乳素（scPRL）和鳙鱼催乳素（bcPRL）的cDNA已被克隆。从核苷酸序列推导可知，scPRL和bcPRL均由187个氨基酸残基组成。scPRL和bcPRL之间只有一个残基不同。同源性分析表明，scPRL和bcPRL与鲤鱼催乳素高度同源（97%），与鲑鱼、鳟鱼和罗非鱼的催乳素相对保守（64 - 69%），与鸟类和哺乳动物的催乳素差异较大（30 - 35%）。与其他鱼类的催乳素类似，scPRL和bcPRL缺乏鸟类和哺乳动物催乳素N端的前12个残基。