Department of Orthopedic Surgery, State University of New York at Buffalo, Buffalo, New York 14214, USA.
J Biomed Mater Res A. 2010 Jun 15;93(4):1500-9. doi: 10.1002/jbm.a.32622.
An electrochemically controlled system has been developed which allows for cell culture directly on electrically polarized metal surfaces with simultaneous control and assessment of the electrochemical current, potential, and impedance of the interface. This system was utilized in this study to assess the interactions between electrochemically polarized commercially pure titanium (cpTi) and MC3T3 preosteoblast cells. Cells were cultured on CpTi for 24 h at static potentials between -1000 mV and +1000 mV vs. Ag/AgCl and cell morphology (SEM and cell area) and viability (MTT and Live-Dead assay) were assessed along with the electrochemical current densities and surface oxide impedance properties. The results indicate that cathodic polarization in the range of -600 mV to -1000 mV markedly reduces the spreading and viability of cells cultured directly on cpTi within 24 h, while anodic polarization (-300 mV to +1000 mV) out to 72 h shows no difference in cell behavior as compared to the OCP condition. Analysis of the relationship between the cell outcomes and the electrochemical current densities and impedance indicated the presence of voltage-dependent electrochemical thresholds (cathodic current density, i(c) > 1.0 microA/cm(2), R(p) < 10(5) Omega cm(2)) which may control the biocompatibility of cpTi. In addition, these outcomes have direct clinical significance for modular orthopedic implants whose potential can shift, via fretting corrosion, down into the range of potentials exhibiting poor cell behavior.
已经开发出一种电化学控制体系，可以直接在电极化金属表面上进行细胞培养，同时控制和评估界面的电化学电流、电位和阻抗。本研究中利用该系统评估了电化学极化商用纯钛 (cpTi) 与 MC3T3 前成骨细胞之间的相互作用。将细胞在静态电位为-1000 mV 至+1000 mV 相对于 Ag/AgCl 的 cpTi 上培养 24 h，评估细胞形态（SEM 和细胞面积）和活力（MTT 和 Live-Dead 测定）以及电化学电流密度和表面氧化物阻抗特性。结果表明，在-600 mV 至-1000 mV 的阴极极化范围内，显著降低了直接在 cpTi 上培养的细胞的铺展和活力，而在 72 h 内的阳极极化（-300 mV 至+1000 mV）与 OCP 条件相比，细胞行为没有差异。对细胞结果与电化学电流密度和阻抗之间的关系进行分析表明，存在电压依赖性电化学阈值（阴极电流密度，i(c) > 1.0 microA/cm(2)，R(p) < 10(5) Omega cm(2))，可能控制 cpTi 的生物相容性。此外，这些结果对于模块化骨科植入物具有直接的临床意义，其电势可能通过微动腐蚀而转移到表现出不良细胞行为的电位范围内。
