Wilson D F
Am J Physiol. 1977 Nov;233(5):C157-63. doi: 10.1152/ajpcell.1977.233.5.C157.
Quantal-release and binomial statistical-release parameters were examined in the isolated rat diaphragm phrenic nerve preparation. The muscle resting potentials were reduced by cutting the muscle fibers to prevent muscle action potentials and contractions. The cutting technique was modified to allow persistent observation of miniature end-plate potentials (MEPPs) and end-plate potentials (EPPs) with intracellular recording techniques. Direct estimates of quantal release were examined and compared with predicted binomial and Poisson distributions. The results indicate that release is binomial when the nerve is stimulated with low- or high-frequency stimuli. The indirect method of estimating quantal release (variance method), which assumes release is described by a Poisson distribution, seriously overestimates quantal release. The binomial analysis indicates that the statistical store is small (less than 90 quanta) and that most of these quanta are released with each nerve impulse.
在离体大鼠膈神经膈肌标本中研究了量子释放和二项式统计释放参数。通过切断肌纤维以防止肌肉动作电位和收缩，从而降低肌肉静息电位。对切断技术进行了改进，以便使用细胞内记录技术持续观察微小终板电位（MEPPs）和终板电位（EPPs）。对量子释放的直接估计进行了检验，并与预测的二项式和泊松分布进行了比较。结果表明，当用低频或高频刺激神经时，释放是二项式的。假定释放由泊松分布描述的估计量子释放的间接方法（方差法）严重高估了量子释放。二项式分析表明，统计储存量较小（少于90个量子），并且这些量子中的大多数在每次神经冲动时都会释放。
