Wilson D F
Am J Physiol. 1979 Jul;237(1):C31-7. doi: 10.1152/ajpcell.1979.237.1.C31.
The characteristics of depression, facilitation, and mobilization of transmitter were examined at the rat diaphragm neuromuscular junction. Intracellular recording techniques were used to monitor end-plate potentials (EPPs), miniature end-plate potentials (MEPPs) and the muscle resting potentials. The cut-muscle technique was used to prevent muscle action potentials. Quantal release was determined by the direct method. The binomial statistical parameters, releasable store (n) and probability of release (p), were examined under various stimulating conditions to determine the basis for depression and facilitation. The present experiments demonstrate that p remains unchanged during repetitive nerve stimulation at low or moderately high frequencies. The experiments demonstrate that depression is due to a decrease in n and facilitation is due to an elevation in n. It is suggested that the increase in n during facilitation is due to a transient recruitment of inactive releasing sites. Substantial replenishment of n by mobilization occurs within a few ms after a stimulus but a slow residual rate of mobilization is needed to replenish n to resting levels.
在大鼠膈神经肌肉接头处研究了递质释放的抑制、易化和动员的特征。采用细胞内记录技术监测终板电位（EPPs）、微小终板电位（MEPPs）和肌肉静息电位。采用肌肉切断技术来防止肌肉动作电位。通过直接方法测定量子释放。在各种刺激条件下检查二项式统计参数、可释放储备（n）和释放概率（p），以确定抑制和易化的基础。目前的实验表明，在低频或中等高频重复神经刺激期间，p保持不变。实验表明，抑制是由于n的减少，易化是由于n的升高。有人提出，易化期间n的增加是由于非活性释放位点的短暂募集。刺激后几毫秒内通过动员对n进行大量补充，但需要缓慢的残余动员速率才能将n补充到静息水平。
