Guo Xiu-tian, Dong Qing-jun, Cao Yong-qing
Department of Anorectal Surgery, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China.
Zhong Xi Yi Jie He Xue Bao. 2009 Dec;7(12):1154-8. doi: 10.3736/jcim20091212.
To observe the effects of mild moxibustion on angiogenesis and microcirculation in wound repair after operation of anal fistula, and to explore the mechanism of action.
Seventy-two SD male rats were made to bear an "acute, open, bleeding, infected" wound surface and were divided randomly into mild moxibustion group, microwave group and control group (24 rats per group). The wound surfaces of the treatment groups were treated for 15 min with mild moxibustion or microwave medical instrument from the first day after operation, and the control group was not given any treatment. At the first, third, seventh and fourteenth days after operation (1, 3, 7 and 14 days of treatment), six rats in each group were sacrificed after examining the blood flow of the wound surface, and the samples of granulation tissues were collected. The numbers of CD34 and positive expression of vascular endothelial growth factor (VEGF) in granulation tissues were detected.
In the early and middle stages of wound healing, mild moxibustion could obviously increase the expression of VEGF, the number of CD34, and the blood flow of the wound surface. And the expression of VEGF, the number of CD34, and the blood flow of the wound surface began to increase at the third day, and reached the peak level at the seventh day, and then began to decrease. In the late stage of wound healing (day 14), mild moxibustion could decrease the expression of VEGF, the number of CD34, and decrease the blood flow of the wound surface. There were significant differences between the mild moxibustion group and the control group in the blood flow of the wound surface, the expression of VEGF and the numbers of CD34 in granulation tissues (P<0.05) after treatment, and except the number of CD34 at 14-day treatment, there were also differences between the mild moxibustion group and the microwave group.
Mild moxibustion has better regulative actions on blood flow of the wound surface and the number of CD34 and positive expression of VEGF in granulation tissues, and can regulate microcirculation in wound surface and promote the wound healing.
观察温和灸对肛瘘术后创面修复中血管生成及微循环的影响，探讨其作用机制。
将72只雄性SD大鼠制成“急性、开放性、出血、感染”创面，随机分为温和灸组、微波组和对照组（每组24只）。治疗组创面于术后第1天开始用温和灸或微波治疗仪治疗15分钟，对照组不给予任何处理。术后第1、3、7、14天（治疗1、3、7、14天），每组处死6只大鼠，检测创面血流情况后，取肉芽组织样本。检测肉芽组织中CD34数量及血管内皮生长因子（VEGF）阳性表达情况。
在创面愈合的早、中期，温和灸能明显增加VEGF表达、CD34数量及创面血流。VEGF表达、CD34数量及创面血流于第3天开始增加，第7天达到峰值，随后开始下降。在创面愈合后期（第14天），温和灸能降低VEGF表达、CD34数量及创面血流。治疗后，温和灸组与对照组在创面血流、VEGF表达及肉芽组织中CD34数量方面有显著差异（P<0.05），除治疗14天时CD34数量外，温和灸组与微波组之间也有差异。
温和灸对创面血流、肉芽组织中CD34数量及VEGF阳性表达有较好的调节作用，能调节创面微循环，促进创面愈合。
