塞内加尔鳎（Solea senegalensis）（Kaup）睾丸的凝集素结合模式。

Department of Animal Health and Well-being, Faculty of Veterinary Medicine, University of Bari, S.P. per Casamassima Km. 3, Bari, Italy.

Histol Histopathol. 2010 Feb;25(2):205-16. doi: 10.14670/HH-25.205.

The localization and characterization of oligosaccharide sequences in the testis of Senegalese sole Solea senegalensis was investigated using 12 lectins in combination with KOH saponification and sialidase digestion (K-s). The interstitial compartment contained all the sugar residues investigated, those bearing oligosaccharides terminating with sialic acid (Neu5Ac) alpha2,3Galbeta1,4GlcNAc, Neu5AcGalNAcalpha1,3(LFucbeta1,2) Galbeta1,3/4GlcNAcbeta1 and GalNAcalpha1,3(LFuc1,2) Galbeta1,3/4GlcNAcbeta1 being more abundant in the medullar region than in the cortex. The melano-macrophage centres found in the interstitial compartment displayed glycans terminating with Galbeta1,3GalNAc. The basal lamina separating the germinal and interstitial compartments exhibited glycans with terminal/internal mannose, internal betaGlcNAc, and terminal Neu5Acalpha2,6Gal/GalNAc, and Neu5AcGalbeta1,3GalNAc, Galbeta1,3GalNAc (PNA), Galbeta1,4GlcNAc, GalNAc, alphaGal, and alphaL-Fuc. In the germinal compartment, the Sertoli cells expressed only glycans terminating with Neu5Acalpha2,3Galbeta1,4GlcNAc in the apical and supra-nuclear lateral surface of the spermatonial cysts located in the distal part of the seminiferous lobules. Primary spermatocytes exhibited oligosaccharides terminating with Galbeta1,3GalNAc and alphaGalNAc in the cytoplasm and nucleus, respectively. The spermatids contained highly mannosylated glycans terminating with GalNac, alphaGal, and alphaL-Fuc. The head of spermatozoa expressed a more complex glycosylation pattern characterized by the additional presence of oligosaccharides terminating with Neu5Acalpha2,3Galbeta1,4GlcNAc, Neu5AcGalbeta1,3GalNAc, Neu5AcGalNAcalpha1,3(LFuca1,2)Galbeta1,3/4GlcNAcbeta1, GalNAcalpha1,3(LFucalpha1,2)Galbeta1,3/4GlcNAcbeta1. The comparison with previous lectin histochemical studies carried out in other fish species reveals a specific glycosylation pattern of Senegalese sole testicular structures and spermatozoa head.

采用 12 种凝集素与 KOH 皂化和唾液酸酶消化（K-s）相结合的方法，研究了塞内加尔比目鱼（Solea senegalensis）睾丸中寡糖序列的定位和特征。间质区含有所有研究的糖残基，那些带有以唾液酸（Neu5Ac）α2,3Galβ1,4GlcNAc、Neu5AcGalNAcα1,3（LFucβ1,2）Galβ1,3/4GlcNAcβ1 和 GalNAcα1,3（LFuc1,2）Galβ1,3/4GlcNAcβ1 末端的寡糖在髓质区比皮质区更为丰富。在间质区发现的黑素巨噬细胞中心显示出以 Galβ1,3GalNAc 为末端的聚糖。将生殖区和间质区分开的基底层表现出末端/内部甘露糖、内部βGlcNAc 和末端 Neu5Aca2,6Gal/GalNAc、Neu5AcGalβ1,3GalNAc、Galβ1,3GalNAc（PNA）、Galβ1,4GlcNAc、GalNAc、αGal 和αL-Fuc 的聚糖。在生殖区，Sertoli 细胞仅在位于精小叶远端的生精囊中位于顶部和核旁侧表面的精子囊中表达以 Neu5Aca2,3Galβ1,4GlcNAc 为末端的聚糖。初级精母细胞在细胞质和核中分别显示以 Galβ1,3GalNAc 和αGalNAc 为末端的寡糖。精子含有高度甘露糖化的聚糖，以 GalNac、αGal 和αL-Fuc 为末端。精子头部表达一种更为复杂的糖基化模式，其特征是还存在以 Neu5Aca2,3Galβ1,4GlcNAc、Neu5AcGalβ1,3GalNAc、Neu5AcGalNAcα1,3（LFucα1,2）Galβ1,3/4GlcNAcβ1、GalNAcα1,3（LFucα1,2）Galβ1,3/4GlcNAcβ1 为末端的寡糖。与在其他鱼类中进行的先前凝集素组织化学研究进行比较，揭示了塞内加尔比目鱼睾丸结构和精子头部的特定糖基化模式。