Desantis S, Acone F, Corriero A, Deflorio M, Zubani D, Ventriglia G, Palmieri G, De Metrio G
Dept. of Animal Health and Well-being, Faculty of Veterinary Medicine, University of Bari, Italy.
Eur J Histochem. 2004 Oct-Dec;48(4):403-12. doi: 10.4081/914.
The distribution of sialic acid residues as well as other glycosidic sugars has been investigated in the horse oviductal isthmus during anoestrus, oestrus and pregnancy by means of lectin and pre-lectin methods. Ciliated cells and non-ciliated (secretory) cells exhibited different lectin binding profiles that were found to change during the investigated stages. Ciliated cells did not show any reactivity in the basal cytoplasm, while the supra-nuclear cytoplasm displayed a few of oligosaccharides with terminal and internal alphamannose (Man) and/or alphaglucose (Glc) during oestrus and pregnancy and a moderate presence of oligosaccharides terminating in alphafucose (Fuc) during oestrus; cilia exhibited a more complex glycoconjugate pattern for the presence of oligosaccharides terminating in N-acetylgalactosamine (GalNAc), GalNAcalpha1,3 GalNAcalpha1,3galactose(Gal)beta1,4Galbeta1,4N-acetylglucosamine(GlcNAc), Fuc, sialic acid (Neu5Ac)-aGalNAc belonging or not to the GalNAca1,3GalNAca1,3 Galb1,4 Galb1, 4GlcNAc sequence, and. alphaGalNAc and Neu5Aca 2,6Gal/GalNAc increased during oestrus. Cilia displayed terminal Galbeta1,3 GalNAc in pregnancy, terminal alphaGal in anoestrus and pregnancy and terminal or internal D-GlcNAc during anoestrus and pregnancy, respectively. The whole cytoplasm of non-ciliated cells showed oligosaccharides terminating with alphaGalNAc, Neu5Aca2,6Gal/GalNAc, Neu5Ac GalNAca 1,3GalNAcalpha1,3Galbeta1,4Galbeta1,4GlcNAc during the investigated stages, as well as GlcNAc in anoestrus and pregnancy. The supra-nuclear zone of non-ciliated cells exhibited oligosaccharides with terminal Galbeta1,4GlcNAc and internal Man during oestrus and pregnancy as well as terminal alphaGal and Fuc in oestrus and Neu5Ac-Galbeta1,3GalNAc in pregnancy. The luminal surface of non-ciliated cells showed glycans terminating with alphaGalNAc and/or Neu5Ac GalNAcalpha1,3 GalNAcalpha1,3Galbeta1,4Galbeta1,4GlcNAc in all specimens, oligosaccharides with terminal Galbeta1,4GlcNAc and internal Man during oestrus and pregnancy, Neu5Ac alpha2,6Gal/GalNAc in anoestrus and oestrus, and glycans terminating with Galbeta1,3GalNAc, Neu5A acalpha2,3 Galbeta1, 4GlcNac, Neu5ac-Galbeta1,3GalNAc, Neu5Ac-Galbeta1,4 GlcNAc in pregnancy. These findings show the presence of sialoglycoconjugates in the oviductal isthmus of the mare as well as the existence of great modifications in the glycoconjugates linked to different physiological conditions.
运用凝集素和前凝集素方法，研究了发情间期、发情期和妊娠期母马输卵管峡部唾液酸残基以及其他糖苷糖的分布情况。纤毛细胞和非纤毛（分泌）细胞呈现出不同的凝集素结合模式，且在研究阶段中会发生变化。纤毛细胞的基底细胞质无任何反应性，而在发情期和妊娠期，核上细胞质显示出一些带有末端和内部α-甘露糖（Man）和/或α-葡萄糖（Glc）的寡糖，发情期有中等数量以α-岩藻糖（Fuc）结尾的寡糖；纤毛因存在以N-乙酰半乳糖胺（GalNAc）、GalNAcα1,3GalNAcα1,3半乳糖（Gal）β1,4Galβ1,4N-乙酰葡糖胺（GlcNAc）、Fuc、唾液酸（Neu5Ac）-αGalNAc结尾的寡糖而呈现出更复杂的糖缀合物模式，αGalNAc和Neu5Acα2,6Gal/GalNAc在发情期增加。纤毛在妊娠期显示末端Galβ1,3GalNAc，发情间期和妊娠期分别显示末端αGal以及末端或内部D-GlcNAc。非纤毛细胞的整个细胞质在研究阶段均显示以αGalNAc、Neu5Acα2,6Gal/GalNAc、Neu5Ac GalNAcα1,3GalNAcα1,3Galβ1,4Galβ1,4GlcNAc结尾的寡糖，发情间期和妊娠期还有GlcNAc。非纤毛细胞的核上区在发情期和妊娠期显示带有末端Galβ1,4GlcNAc和内部Man的寡糖，发情期还有末端αGal和Fuc，妊娠期有Neu5Ac-Galβ1,3GalNAc。非纤毛细胞的管腔表面在所有样本中均显示以αGalNAc和/或Neu5Ac GalNAcα1,3 GalNAcα1,3Galβ1,4Galβ1,4GlcNAc结尾 的聚糖，发情期和妊娠期有带有末端Galβ1,4GlcNAc和内部Man的寡糖，发情间期和发情期有Neu5Acα2,6Gal/GalNAc，妊娠期有以Galβ1,3GalNAc、Neu5Acα2,3 Galβ1, 4GlcNac、Neu5ac-Galβ1,3GalNAc、Neu5Ac-Galβ1,4 GlcNAc结尾的聚糖。这些发现表明母马输卵管峡部存在唾液酸糖缀合物，以及与不同生理状况相关的糖缀合物存在重大变化。
