Departamento de Microbiología y Parasitología, Universidad de Alcalá, Ctra. Madrid-Barcelona, Madrid, Spain.
Bioresour Technol. 2010 Apr;101(7):2224-9. doi: 10.1016/j.biortech.2009.11.061. Epub 2009 Dec 16.
The ability of a laccase (EC 1.10.3.2) produced by Streptomyces cyaneus CECT 3335 to decolourise and detoxify azo dyes was assessed. Results showed that a colour loss of 90% was achieved only in the presence of acetosyringone (0.1mM) acting as a redox mediator for the laccase. Toxicological analysis of the decolourised dyes revealed that there was no direct correlation between decolouration and detoxification; in fact, in the case of the dyes Methyl Orange and Orange II, a significant increase in toxicity was produced after the treatment. In contrast, a significant decrease in toxicity was observed after the decolouration of New Coccine and Chromotrope 2R. Finally, HPLC analysis of the dyes after treatment revealed the complete disappearance of both dyes and mediator and a concomitant appearance of new chromatographic peaks which could be responsible of the residual toxicity detected in some cases.
评估了链霉菌（Streptomyces cyaneus）CECT 3335 产生的漆酶（EC 1.10.3.2）对偶氮染料的脱色和解毒能力。结果表明，只有在作为漆酶氧化还原介体的乙酰丁香酮（0.1mM）存在的情况下，才能达到 90%的颜色损失。对脱色染料的毒理学分析表明，脱色和解毒之间没有直接的相关性；事实上，对于甲基橙和橙色 II 染料，处理后毒性显著增加。相比之下，新茜素和铬变酸 2R 脱色后，毒性显著降低。最后，处理后染料的 HPLC 分析表明，两种染料和介体完全消失，同时出现新的色谱峰，这可能是在某些情况下检测到残留毒性的原因。
