Chemical Engineering, Queen's University, Kingston, Ontario, Canada K7L 3N6.
Bioresour Technol. 2010 Apr;101(7):2230-5. doi: 10.1016/j.biortech.2009.11.066. Epub 2009 Dec 16.
The decolorization and detoxification of textile dyes by fungal laccase immobilized on porous glass beads were evaluated. Anthraquinone (Reactive blue 19 and Dispersed blue 3) and indigoid (Acid blue 74) dyes were degraded more rapidly than the azo dyes (Acid red 27 and Reactive black 5). There was no dye sorption to the enzyme bed when decolorization rates were high (>12 microM dye/U-h) but at moderate rates (8 to>0.06 microM/U-h), there was a transient color which disappeared upon prolonged exposure. With Reactive black 5, permanent adsorption occurred most likely because laccase had been totally inactivated. Although laccase treatment was more efficient at decolorizing the anthraquinone dyes, their toxicity (as determined by the Microtox assay) increased while the less efficiently decolorized solutions of azo and indigoid dyes became less toxic. These results demonstrate the potential and limitations of using immobilized laccase to enzymatically decolorize a range of different dye classes and reduce dye toxicity in a single step.
评估了固定在多孔玻璃珠上的真菌漆酶对纺织染料的脱色和解毒作用。蒽醌（活性蓝 19 和分散蓝 3）和吲哚（酸性蓝 74）染料比偶氮染料（酸性红 27 和活性黑 5）降解得更快。当脱色速率较高（>12 μM 染料/U-h）时，酶床上没有染料吸附，但在中等速率（8 至>0.06 μM/U-h）下，存在暂时的颜色，延长暴露时间后会消失。对于活性黑 5，可能由于漆酶已完全失活而发生永久性吸附。尽管漆酶处理对蒽醌染料的脱色效果更好，但它们的毒性（通过 Microtox 测定）增加了，而那些较难脱色的偶氮和吲哚染料的溶液毒性则降低了。这些结果表明，使用固定化漆酶在单一步骤中酶促脱色一系列不同染料类别并降低染料毒性具有潜力和局限性。
