Trinkaus-Randall V, Leibowitz H M, Ryan W J, Kupferman A
Department of Ophthalmology, Boston University School of Medicine, MA 02118.
Invest Ophthalmol Vis Sci. 1991 Mar;32(3):603-9.
An intrastromal injection of endotoxin lipopolysaccharide (LPS) in one eye of New Zealand albino rabbits induced a prominent keratitis characterized clinically and microscopically by edema and infiltration. Polymorphonuclear leukocytes (PMNs) constituted the primary invading leukocytic element. Collagen synthesis was measured by pulsing the corneas with 3H-proline before inducing inflammation. The invasion of the cornea by leukocytes did not alter the conversion of proline to hydroxyproline significantly in the stroma during the 14-day observation period, signifying that there were only negligible changes in the rate of collagen synthesis. However, the percentage of total stromal protein represented by collagen (ie, collagen/total protein) was only 50% of that in comparable corneas receiving an injection of phosphate-buffered saline. Some animals were rendered leukopenic by intravenous nitrogen mustard before intrastromal LPS injection caused a less severe corneal inflammatory response, characterized microscopically by fewer infiltrating leukocytes. Similarly, in nonleukopenic rabbits, topical therapy with 1% prednisolone acetate markedly reduced the corneal inflammatory response which also was characterized by fewer invading leukocytes. In neither instance was there extreme collagen loss, suggesting that the loss of stromal collagen is related to PMN infiltration.
在新西兰白化兔的一只眼睛的基质内注射内毒素脂多糖（LPS）可诱发显著的角膜炎，在临床和显微镜下表现为水肿和浸润。多形核白细胞（PMN）是主要的侵入性白细胞成分。在诱发炎症之前，通过用³H - 脯氨酸脉冲标记角膜来测量胶原蛋白合成。在14天的观察期内，白细胞对角膜的侵入并未显著改变基质中脯氨酸向羟脯氨酸的转化，这表明胶原蛋白合成速率仅有微不足道的变化。然而，胶原蛋白占总基质蛋白的百分比（即胶原蛋白/总蛋白）仅为接受磷酸盐缓冲盐水注射的对照角膜的50%。在基质内注射LPS之前，一些动物通过静脉注射氮芥造成白细胞减少，其角膜炎症反应较轻，显微镜下表现为浸润的白细胞较少。同样，在非白细胞减少的兔子中，局部应用1%醋酸泼尼松龙可显著减轻角膜炎症反应，其特征同样是侵入的白细胞较少。在这两种情况下均未出现严重的胶原蛋白丢失，提示基质胶原蛋白的丢失与PMN浸润有关。
