串联翻译始于大肠杆菌的cheA基因座。
Tandem translation starts in the cheA locus of Escherichia coli.
作者信息
Kofoid E C, Parkinson J S
机构信息
Biology Department, University of Utah, Salt Lake City 84112.
出版信息
J Bacteriol. 1991 Mar;173(6):2116-9. doi: 10.1128/jb.173.6.2116-2119.1991.
Abstract
The cheA locus of Escherichia coli encodes two protein products, CheAL and CheAS. The nucleotide sequences of the wild-type cheA locus and of two nonsense alleles confirmed that both proteins are translated in the same reading frame from different start points. These start sites were located on the coding sequence by direct determination of the amino-terminal sequences of the two CheA proteins. Both starts are flanked by inverted repeats that may play a role in regulating the relative expression rates of the CheA proteins through alternative mRNA secondary structures.
摘要
大肠杆菌的cheA基因座编码两种蛋白质产物，即CheAL和CheAS。野生型cheA基因座和两个无义等位基因的核苷酸序列证实，这两种蛋白质是从不同的起始点以相同的阅读框进行翻译的。通过直接测定两种CheA蛋白的氨基末端序列，确定了这些起始位点位于编码序列上。两个起始位点均侧翼有反向重复序列，这些反向重复序列可能通过替代mRNA二级结构在调节CheA蛋白的相对表达率中发挥作用。
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