Biochemistry of Membranes, Bijvoet Center for Biomolecular Research, Utrecht University, 3584CH Utrecht, The Netherlands.
Eur Biophys J. 2010 Mar;39(4):609-21. doi: 10.1007/s00249-009-0567-1. Epub 2009 Dec 18.
In this review we discuss recent insights obtained from well-characterized model systems into the factors that determine the orientation and tilt angles of transmembrane peptides in lipid bilayers. We will compare tilt angles of synthetic peptides with those of natural peptides and proteins, and we will discuss how tilt can be modulated by hydrophobic mismatch between the thickness of the bilayer and the length of the membrane spanning part of the peptide or protein. In particular, we will focus on results obtained on tryptophan-flanked model peptides (WALP peptides) as a case study to illustrate possible consequences of hydrophobic mismatch in molecular detail and to highlight the importance of peptide dynamics for the experimental determination of tilt angles. We will conclude with discussing some future prospects and challenges concerning the use of simple peptide/lipid model systems as a tool to understand membrane structure and function.
在这篇综述中，我们讨论了从经过良好表征的模型系统中获得的最新见解，这些见解涉及决定跨膜肽在脂质双层中取向和倾斜角度的因素。我们将比较合成肽和天然肽与蛋白质的倾斜角度，并讨论如何通过双层厚度与肽或蛋白质跨膜部分长度之间的疏水失配来调节倾斜。特别地，我们将重点讨论在色氨酸侧翼模型肽（WALP 肽）上获得的结果，作为一个案例研究，以详细说明疏水失配对分子的可能后果，并强调肽动力学对于实验确定倾斜角度的重要性。最后，我们将讨论使用简单的肽/脂质模型系统作为理解膜结构和功能的工具的一些未来前景和挑战。
