1型糖尿病患儿外周血单个核细胞中晚期糖基化终产物（AGEs）及其受体（RAGE）的可溶性受体（sRAGE）血清水平之间的关联。

Association between serum levels of the soluble receptor (sRAGE) for advanced glycation endproducts (AGEs) and their receptor (RAGE) in peripheral blood mononuclear cells of children with type 1 diabetes mellitus.

作者信息

Dettoraki Athina, Gil Andrea Paola Rojas, Spiliotis Bessie E

机构信息

Division of Pediatric Endocrinology and Diabetes, Department of Pediatrics, University of Patras, School of Medicine, Patras, Greece.

出版信息

J Pediatr Endocrinol Metab. 2009 Oct;22(10):895-904. doi: 10.1515/jpem.2009.22.10.895.

DOI:10.1515/jpem.2009.22.10.895

PMID:20020577

Abstract

AIM

The binding of AGEs to RAGE is involved in diabetic vascular complications. We studied sRAGE levels and RAGE protein expression (P) together with N-carboxymethyl lysine (CML), a major AGE, in 74 patients with type 1 diabetes mellitus (DM1) and 43 healthy (C) children.

METHODS

sRAGE and CML levels were determined by ELISA and RAGE P was evaluated in mononuclear cells by Western immunoblotting.

RESULTS

Serum sRAGE was higher in DM1 than in C (1430 +/- 759 vs 1158 +/- 595 pg/ml, p = 0.047), inversely correlated to diabetes duration (r = -0.265, p = 0.037) and directly correlated to LDL-cholesterol levels (r = 0.224, p = 0.039). Diabetes duration correlated independently with sRAGE (p = 0.034). Circulating CML levels were not significantly different between DM1 and C groups (3.51 +/- 1.49 vs 3.59 +/- 1.83 ng/ml, p > 0.05) and RAGE P was lower in DM1 than in C (61 +/- 46 vs 102 +/- 63%, p = 0.0001).

CONCLUSIONS

Increased serum sRAGE in children with DM1 may provide temporary protection against cell damage and may be sufficient to eliminate excessive circulating CML.

摘要

目的

晚期糖基化终末产物（AGEs）与晚期糖基化终末产物受体（RAGE）的结合参与糖尿病血管并发症的发生。我们研究了74例1型糖尿病（DM1）患儿和43例健康（C）儿童的可溶性RAGE（sRAGE）水平、RAGE蛋白表达（P）以及主要AGE——N-羧甲基赖氨酸（CML）的情况。

方法

采用酶联免疫吸附测定法（ELISA）测定sRAGE和CML水平，通过Western免疫印迹法评估单核细胞中的RAGE P。

结果

DM1患儿血清sRAGE水平高于C组儿童（1430±759 vs 1158±595 pg/ml，p = 0.047），与糖尿病病程呈负相关（r = -0.265，p = 0.037），与低密度脂蛋白胆固醇水平呈正相关（r = 0.224，p = 0.039）。糖尿病病程与sRAGE独立相关（p = 0.034）。DM1组和C组的循环CML水平无显著差异（3.51±1.49 vs 3.59±1.83 ng/ml，p>0.05），DM1组的RAGE P低于C组（61±46 vs 102±63%，p = 0.0001）。